<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Dear Chun-Nan Chen,<div class="">As Tom Goddard suggested, it is recommended to use ChimeraX instead if you have surface calculation errors in Chimera.<br class=""><div class=""><br class=""></div><div class="">However, if this is for commercial use, both programs require licensing:</div><div class=""><br class=""></div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimerax/docs/licensing.html" class="">http://www.rbvi.ucsf.edu/chimerax/docs/licensing.html</a>></div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/licensing.html" class="">http://www.rbvi.ucsf.edu/chimera/docs/licensing.html</a>></div><div class=""><br class=""></div><div class="">It may be different on your computer, but when I use Chimera to show surface on 6VSB, the outer surface of chain B is displayed even though there is an error message. At least on my laptop, the failure is on some interior bubble that is not needed to display the outer surface; in the attached image, chain A is dodger blue, B is yellow, C is red. If you don't have any surface on chain B on your computer after the error, however, the best advice is to use ChimeraX instead.</div><div class=""><br class=""></div><div class="">I hope this helps,</div><div class="">Elaine</div><div class=""><div class="">-----<br class="">Elaine C. Meng, Ph.D. <br class="">UCSF Chimera(X) team<br class="">Department of Pharmaceutical Chemistry<br class="">University of California, San Francisco<br class=""></div><div class=""><br class="webkit-block-placeholder"></div><div class=""><img apple-inline="yes" id="0F6749E3-FFD1-4A3E-8153-0071B14E28BB" width="640" height="425" src="cid:207117C6-2DAF-40B9-A0CB-88A1324D5E4D@gateway.sonic.net" class=""></div><blockquote type="cite" class="">On Apr 18, 2020, at 11:53 PM, Chun-Nan Chen <<a href="mailto:chunnan.chen@singlecelltechnology.com" class="">chunnan.chen@singlecelltechnology.com</a>> wrote:<br class=""><br class="">Hello,<br class=""> <br class="">I apologize. The PDB no. should be 6VSB. Thank you for your assistance.<br class=""> <br class="">Chun-Nan Chen, Ph.D.<br class="">CEO<br class="">Single Cell Technology, Inc.<br class="">6280 San Ignacio Ave. Suite E<br class="">San Jose, CA 95119<br class="">Mobile: (408) 642-9741<br class="">Email: <a href="mailto:chunnan.chen@singlecelltechnology.com" class="">chunnan.chen@singlecelltechnology.com</a><br class="">Web: singlecelltechnology.com<br class=""> <br class=""> <br class=""> <br class="">From: Chun-Nan Chen <chunnan.chen@singlecelltechnology.com> <br class="">Sent: Saturday, April 18, 2020 10:45 PM<br class="">To: 'chimera-users@cgl.ucsf.edu' <chimera-users@cgl.ucsf.edu><br class="">Subject: Visualizing SARS-CoV-2 spike protein<br class=""> <br class="">Hello,<br class=""> <br class="">I am trying to visualize the SARS-CoV-2 spike protein, which is a trimer (PDB no. 6CRZ). I wish to surface chain B, which is in an “up” conformation) but it kept giving me an error message. I tried the “split” command and other solutions without success. I could surface just the B chain but I really want to surface all 3 chains and color the RBD.<br class=""> <br class="">Could anyone help me with this issue?<br class=""> <br class="">Thank you,<br class=""> <br class="">Chun-Nan Chen, Ph.D.<br class="">CEO<br class="">Single Cell Technology, Inc.<br class="">6280 San Ignacio Ave. Suite E<br class="">San Jose, CA 95119<br class="">Mobile: (408) 769-2171<br class="">Email: chunnan.chen@singlecelltechnology.com<br class="">Web: singlecelltechnology.com<br class=""> <br class="">_______________________________________________<br class="">Chimera-users mailing list: Chimera-users@cgl.ucsf.edu<br class="">Manage subscription: http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users<br class=""></blockquote><br class=""></div></div></body></html>