<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="WordSection1" style="page: WordSection1; caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Dear Chimera team,<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">I am trying to display color the surface of my molecule according with Poisson-Boltzmann electrostatics using Chimera 1.14. I ran the PDB2PQR tool as described in the user guide and obtained a PDB2PQR file. With that, I ran the APBS tool and obtained a .dx file. As described, the Surface Color panel opens automatically, but here I am unable to choose my structure file for "color surface" (s. attachment 1). I can only choose the .dx file, which does not work. All three models obtained during this process are opened in the model panel though (s. attachment 2).<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Do you have any idea on how to fix this?<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Thank you very much in advance and keep up the great work!<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Benedikt Moissl<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 8pt; font-family: Verdana, sans-serif;" class="">____________________________________________________________<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 7pt; font-family: Verdana, sans-serif;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Institute of Biochemistry, Genetics & Microbiology<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Department of Microbiology & Archaea Center<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">Grohmann Lab – Single-molecule Biochemistry (</span><span lang="DE" class=""><a href="https://www.uni-regensburg.de/biologie-vorklinische-medizin/mikrobiologie/index.html" style="color: rgb(149, 79, 114); text-decoration: underline;" class=""><span lang="EN-US" style="font-size: 10pt; font-family: Verdana, sans-serif; color: rgb(5, 99, 193);" class="">Homepage</span></a></span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class="">)<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 5pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="DE" style="font-size: 8pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127);" class="">University of Regensburg | Universitätsstraße 31 | D-93053 Regensburg | Room:<span class="Apple-converted-space"> </span></span><span lang="DE" style="font-size: 8pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127); background-color: white; background-position: initial initial; background-repeat: initial initial;" class="">WN_E3._2.323</span><span lang="DE" style="font-size: 9pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127); background-color: white; background-position: initial initial; background-repeat: initial initial;" class=""><span class="Apple-converted-space"> </span>|<span class="Apple-converted-space"> </span></span><span lang="DE" style="font-size: 8pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127);" class="">Phone: +49 941 943 3184 | Fax: +49 941 943 2403 | Mail:<span class="Apple-converted-space"> </span></span><span lang="DE" class=""><a href="mailto:benedikt.moissl@ur.de" style="color: rgb(149, 79, 114); text-decoration: underline;" class=""><span style="font-size: 8pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127);" class="">benedikt.moissl@ur.de</span></a></span><span lang="DE" style="font-size: 8pt; font-family: Verdana, sans-serif; color: rgb(127, 127, 127);" class=""><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="DE" class=""><o:p class=""> </o:p></span></div></div><img style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; 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