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<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Hi Chimera Users<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">This is 2<sup>nd</sup> send to you after I enrolled as a CHIMERA user
</span><span style="font-size:12.0pt;font-family:Wingdings">J</span><span style="font-size:12.0pt">, thanks.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">I need to calculate a radial distance plot from a surface representation of an Adeno Associated Virus structure as shown from literature below.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">However the:<o:p></o:p></span></p>
<ol style="margin-top:0in" start="1" type="1">
<li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo1"><span style="font-size:12.0pt">Action, Surface, Show<o:p></o:p></span></li></ol>
<p class="MsoNormal"><span style="font-size:12.0pt">command fails, reporting CHIMERA often fails for large structures ( AAV is easily over 200K atoms ) with multiple chain IDs ( I have 60 individual chain IDs for each of the 60 capsid proteins ).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Other CHIMERA popup suggestions did not resolve the problem either ( changing molecular surface parameters in the Inspect Selection ( actually didn’t see anything to change ) or in PREFERENCES - NEW SURFACES.
I tried using CHIMERA, Structure editing, change chain IDs to all chain A, but that was disallowed.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Summary: could not figure out how to get CHIMERA to calculate and display a surface representation of an AAV virus and then use that display to calculate radial coloring as shown below.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Any solutions greatly appreciated and thanks.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Rob<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><img width="242" height="225" style="width:2.5166in;height:2.3416in" id="_x0000_i1025" src="cid:image001.jpg@01D56FEE.1FF9C5A0"></span><img width="55" height="196" style="width:.575in;height:2.0416in" id="Picture_x0020_2" src="cid:image002.jpg@01D56FEE.1FF9C5A0"><span style="font-size:12.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;color:black"><br>
</span><span style="font-size:10.0pt;color:black;background:white">_________________________</span><span style="font-size:10.0pt;color:black"><br>
<br>
Robert Gampe, MS | Senior Scientist | RDRU Structural Biology| Pfizer Inc. <o:p>
</o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:black">7030 Kit Creek Rd, Morrisville, NC 27560<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:black">Office: 919-666-5969|</span><span style="font-size:10.0pt;color:gray">
</span><u><span style="font-size:10.0pt;color:blue"><a href="mailto:Robert.Gampe@pfizer.com"><span style="color:blue">Robert.Gampe@pfizer.com</span></a><o:p></o:p></span></u></p>
<p class="MsoNormal"><img border="0" width="242" height="56" style="width:2.525in;height:.5833in" id="Picture_x0020_1" src="cid:image003.jpg@01D56FEE.1FF9C5A0" alt="cid:image006.jpg@01D4D043.EB786830"><span style="font-size:10.0pt;color:#1F497D"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:black;background:white">_________________________</span><span style="font-size:10.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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