<div dir="auto">Thank you for the feedback.<div dir="auto"><br></div><div dir="auto">Could you go into more description as to how to decide and choose which residues are polar and nonpolar. </div><div dir="auto"><br></div><div dir="auto">Thank you,</div><div dir="auto">Bailey </div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, Sep 16, 2019, 3:52 PM Elaine Meng <<a href="mailto:meng@cgl.ucsf.edu">meng@cgl.ucsf.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi Bailey,<br>
You will have to decide for yourself which residues you want to consider as polar and nonpolar. Then after you show the surface of the protein, you can select any subset of the residues (or atoms) and then sum the surface area from that subset using the Attribute Calculator tool.<br>
<br>
This is described in more detail in this recent post. Skip down to the fourth paragraph, starting "However, you can get the total surface area given some range of hydrophobicity values…”<br>
<<a href="http://plato.cgl.ucsf.edu/pipermail/chimera-users/2019-September/016140.html" rel="noreferrer noreferrer" target="_blank">http://plato.cgl.ucsf.edu/pipermail/chimera-users/2019-September/016140.html</a>><br>
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Alternatively, you could take a look at the GetArea web server mentioned in this follow-up:<br>
<<a href="http://plato.cgl.ucsf.edu/pipermail/chimera-users/2019-September/016141.html" rel="noreferrer noreferrer" target="_blank">http://plato.cgl.ucsf.edu/pipermail/chimera-users/2019-September/016141.html</a>><br>
<br>
(I changed the subject line of the message to something more descriptive to help others find it later.)<br>
I hope this helps,<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D.<br>
UCSF Chimera(X) team<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<br>
> On Sep 16, 2019, at 12:23 PM, Bailey Onken <<a href="mailto:onken031@umn.edu" target="_blank" rel="noreferrer">onken031@umn.edu</a>> wrote:<br>
> <br>
> Hello,<br>
> I am currently a student in Biochemistry. I have a protein and I am trying to separate polar and nonpolar residues located on the surface of these molecules and then finding the values for the surface area associated with polar and nonpolar areas. If you can help me get to this function I would be very grateful. <br>
> Thank you very much, <br>
> Bailey <br>
<br>
</blockquote></div>