<div dir="ltr"><img width="0" height="0" class="mailtrack-img" alt="" style="display:flex" src="https://mailtrack.io/trace/mail/b9f7204fbf849f5c9566c7aa03f3a635e7346532.png?u=2182203"><br>Hi All, <div><br></div><div>I want to estimate Helical parameter ( Helical Rise, Twist, asymmetric sub unit, per turn  ) of Tau filament for Reference Module generation using Chimera. Is it possible to estimate from raw micrographs or 2D Class Average  by Chimera? </div><div><br></div><div>I was wondering if any of you can provide me procedure if any, will be a great help. Eagerly waiting for your reply.</div><div><br></div><div>Thanks in Advance, </div><div>Reja </div><div> <br></div><div><br></div><div> <br><br><br><div class="mt-signature">
        <table border="0" cellpadding="8" cellspacing="0" style="user-select: none;">
            <tbody><tr>
                <td>
                    <a href="https://mailtrack.io?utm_source=gmail&utm_medium=signature&utm_campaign=signaturevirality5&" class="" style="text-decoration:none">
                        <img src="https://s3.amazonaws.com/mailtrack-signature/sender_notified.gif" alt="Mailtrack" class="" width="32" height="32">
                    </a>
                </td>
                <td>
                    <span style="color:#777">Sender notified by</span> <br>
                    <a href="https://mailtrack.io?utm_source=gmail&utm_medium=signature&utm_campaign=signaturevirality5&" class="mt-install" style="color:#4374f7">Mailtrack</a>
                    <span style="color:transparent;font-size:0">04/14/19, 8:11:49 PM</span>
                </td>
                <td>
                    
                </td>
            </tr>
        </tbody></table>
    </div></div></div><img src="https://my-email-signature.link/signature.gif?u=185991&e=52978676&v=b69523dddc1a32637038fe0adf64c253eca7066c33e79d567095d2f1b3b84242" style="width:0;max-height:0;overflow:hidden">