<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<style type="text/css" style="display:none;"><!-- P {margin-top:0;margin-bottom:0;} --></style>
</head>
<body dir="ltr">
<div id="divtagdefaultwrapper" style="font-size:12pt;color:#000000;font-family:Calibri,Helvetica,sans-serif;" dir="ltr">
<p><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 12pt;">Dear Developers,</span><br>
</p>
<div style="color: rgb(0, 0, 0);">
<div>
<div id="divtagdefaultwrapper" dir="ltr" style="font-size:12pt; color:#000000; font-family:Calibri,Helvetica,sans-serif">
<p>I am try to use Chmiera for electrostatic calculations. I first make the PQR file using the PDB2PQR at different pH, however I do not see a change in the total charge, the REMARK section shows that total charge is 1 always. I'm using the default setting
as shown in the attached screenshot. Please let me know what might be going wrong? I installed the new version twice.</p>
<p><br>
</p>
<p><br>
</p>
<p>Thanks a lot for your help!</p>
<p>Regards</p>
<p>Sowmya</p>
<p><br>
</p>
<p><img naturalheight="841" naturalwidth="657" size="80063" id="img681611" tabindex="0" style="max-width: 99.9%; user-select: none;" src="cid:cdf013bd-01d5-4d3d-9dd2-554c27674417"><br>
</p>
</div>
</div>
</div>
</div>
</body>
</html>