<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Cao,<div class=""><br class=""></div><div class=""> Chimera Gaussian filtering is done by the “vop gaussian” command described in the Chimera manual</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span><a href="https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#gaussian" class="">https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#gaussian</a></div><div class=""><br class=""></div><div class="">It convolves a 3d Gaussian function with a map, a very standard operation that smooths data. It is implemented with a fast Fourier transform. Look up “gaussian convolution” online to find more details about the math, for example,</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span><a href="https://en.wikipedia.org/wiki/Gaussian_blur" class="">https://en.wikipedia.org/wiki/Gaussian_blur</a></div><div class=""><br class=""></div><div class=""> Tom</div><div class=""><br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Dec 12, 2018, at 11:35 PM, CAO, Sheng <<a href="mailto:1155083065@link.cuhk.edu.hk" class="">1155083065@link.cuhk.edu.hk</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div id="divtagdefaultwrapper" dir="ltr" style="caret-color: rgb(0, 0, 0); font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;" class=""><p style="margin-top: 0px; margin-bottom: 0px;" class=""></p><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class="">Dear Sir/Madam,</div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><br class=""></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class="">I am a Ph.D student from <span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class="">The Chinese University of Hong Kong that have just begun to learn structural biology.</span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class=""><br class=""></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class="">I found that the Gaussion filtering in Chimera has a much better result than other programs. And I an quite interested in it.</span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class=""><br class=""></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class="">Could you please share the principle of the <span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class="">Gaussion filtering <span class="">algorithm used in Chimera?</span></span></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class=""><span class=""><br class=""></span></span></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; font-size: 14px;" class=""><span class="">Thank you.</span></span></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><br class=""></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><br class=""></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">Best Regards,</span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">Cao Sheng</span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><br class=""></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><br class=""></span></div><div style="font-family: "lucida Grande", Verdana, "Microsoft YaHei"; font-size: 14px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">Postgraduate of The Chinese University of Hong Kong</span><br style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">Major: Chemistry</span><br style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">E-mail: <a href="mailto:904323223@qq.com" rel="noopener" target="_blank" id="LPlnk100909" class="OWAAutoLink" previewremoved="true" style="outline: none; cursor: pointer; color: rgb(0, 102, 153);">904323223@qq<wbr class="">.com</a></span><br style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">Address: G55, Science Centre South Block, The Chinese University of Hong Kong, Shatin</span><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">, </span><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">New Territories, </span><span style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class="">Hong Kong</span><br style="font-family: Helvetica, "Microsoft Yahei", verdana; line-height: 23.324px;" class=""><span style="font-family: Helvetica, "Microsoft Yahei", verdana; 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