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--></style></head><body lang=EN-US link=blue vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Hi Elaine,</p><p class=MsoNormal>Thank you for your response! I tried using the command and I have been trying two maps which looks very similar, but with different resolution (3.6 A (yellow) vs 3.2 A (grey)) and with different pixel size (1.05 vs 0.822). Will this affect the gridding? (I am not very familiar with what you mean by the same grid). </p><p class=MsoNormal>Best,</p><p class=MsoNormal>Yangqi</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Sent from <a href="https://go.microsoft.com/fwlink/?LinkId=550986">Mail</a> for Windows 10</p><p class=MsoNormal><o:p> </o:p></p><div style='mso-element:para-border-div;border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal style='border:none;padding:0in'><b>From: </b><a href="mailto:meng@cgl.ucsf.edu">Elaine Meng</a><br><b>Sent: </b>Friday, November 16, 2018 7:45 PM<br><b>To: </b><a href="mailto:yangqi.gu@yale.edu">Yangqi Gu</a><br><b>Cc: </b><a href="mailto:chimera-users@cgl.ucsf.edu">chimera-users</a><br><b>Subject: </b>Re: [Chimera-users] Difference map</p></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Hi Yangqi,</p><p class=MsoNormal>Yes, see command “vop subtract”:</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#subtract></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Keep in mind that if the two aren’t on the same grid, there may be small differences due to the need to sample them at the same points to do the subtractions.</p><p class=MsoNormal>I hope this helps,</p><p class=MsoNormal>Elaine</p><p class=MsoNormal>-----</p><p class=MsoNormal>Elaine C. Meng, Ph.D. </p><p class=MsoNormal>UCSF Chimera(X) team</p><p class=MsoNormal>Department of Pharmaceutical Chemistry</p><p class=MsoNormal>University of California, San Francisco</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>> On Nov 16, 2018, at 4:39 PM, Yangqi Gu <yangqi.gu@yale.edu> wrote:</p><p class=MsoNormal>> </p><p class=MsoNormal>> Dear Chimera users,</p><p class=MsoNormal>> I am trying to examine two cryo-EM maps and want to know if there is a subtle change between these two. I want to calculate the difference map, is it doable in Chimera?</p><p class=MsoNormal>> Best,</p><p class=MsoNormal>> Yangqi</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>