<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Hi Yang,<div class="">The standard ribbon for amino acids centers on CA, and as you can see, the CA atoms (alpha-carbons) are towards the lower side of the contour surface in your image.</div><div class=""><br class=""></div><div class="">You can define a ribbon class that uses C (carbonyl carbon) instead of the CA, which will be shifted upward in the orientation shown in your image. Use menu: Tools… Depiction… Ribbon Style Editor, and click on the “Residue Class” tab. The classes should include “amino acid” which is the standard one for amino acids, with CA as the guide atom. Then you can change guide atom from CA to C and then click Apply to see if it is good enough for you. I see it makes a helix but narrower than the one using CA, so it might not be good enough.</div><div class=""><br class=""></div><div class="">In Ribbon Style Editor, if you might want to use that new class later, you can Save to a new name (since you don’t want to overwrite the regular “amino acid” one). Then later in the session you can use command “ribclass” to use that class.</div><div class=""><br class=""></div><div class="">Ribbon Style Editor:<br class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/ribbonstyle/ribbonstyle.html" class="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/ribbonstyle/ribbonstyle.html</a>><br class="">…residue classes:<br class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/ribbonstyle/ribbonstyle.html#class" class="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/ribbonstyle/ribbonstyle.html#class</a>></div><div class="">…ribclass command:</div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/ribclass.html" class="">http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/ribclass.html</a>></div><div class=""><br class=""></div><div class="">For example, I started with “amino acid”, changed guide atom to C, and then saved a new class named “shifted”. Then later without showing the Ribbon Style Editor again, I can just use the new class with a command like:</div><div class=""><br class=""></div><div class="">ribclass shifted #1</div><div class=""><br class=""></div><div class="">… or change it back to default:</div><div class=""><br class=""></div><div class="">ribclass “amino acid” #1</div><div class=""><br class=""></div><div class="">In the image attached below, the tan ribbon is the default one, the blue one is the one using C as guide atom. Using N shifts in the opposite direction </div><div class=""><br class=""></div><div class="">I hope this helps,</div><div class="">Elaine</div><div class=""><div class="">-----<br class="">Elaine C. Meng, Ph.D. <br class="">UCSF Chimera(X) team<br class="">Department of Pharmaceutical Chemistry<br class="">University of California, San Francisco<br class=""><br class=""><img apple-inline="yes" id="6A890680-752F-4842-98C4-DCBA25C6D6A5" src="cid:D0153BE2-BD1C-4A56-8F32-0347D87FA652@cgl.ucsf.edu" class=""></div><blockquote type="cite" class="">On Aug 10, 2018, at 12:35 PM, 师扬 <<a href="mailto:sy31802@163.com" class="">sy31802@163.com</a>> wrote:<br class=""><br class="">Dear chimera developers and users,<br class="">Recently, I met a very strange issue about displaying coordinates in chimera. I found the helices were off-centered with the map, when I open a well refined PDB file and the map it was refined against, and keep an eye on the helices which is automatically displayed by Ribbon. But actually they are will aligned in COOT. And if I hide Ribbon and showed Atoms/Bonds, they were well-aligned. The attached files show the same region with different displaying modes. And I have tried several versions of chimera, the results were the same.<br class="">Actually, I want to use Ribbon to display these helices. Does anyone know how to fix it?<br class="">Thanks in advance!<br class="">Yang<br class=""></blockquote><br class=""></div></body></html>