<div dir="ltr">I open the structures in the first image, which have the secondary structures displayed correctly. I click file save pdb as single file.That's the process I follow to save the file. How can I fix it?</div><div class="gmail_extra"><br><div class="gmail_quote">On Wed, Jun 27, 2018 at 4:54 PM, Elaine Meng <span dir="ltr"><<a href="mailto:meng@cgl.ucsf.edu" target="_blank">meng@cgl.ucsf.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi Ahmad,<br>
I don’t what process you used to save the structures in a single file, so I don’t know what went wrong. All I can tell from your images is that in the bottom image, some of the copies do not have secondary structure assignments, and that is why they show up as thin “coil” ribbon instead of the wider helix and strand ribbon. It might be that something is wrong about the format of your single file, or that some of the backbone atoms are missing. If you don’t think there are missing backbone atoms, you could try command “ksdssp” to reassign helix and strand, but the assignments probably won’t be exactly the same as from the original PDB file.<br>
<<a href="http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/ksdssp.html" rel="noreferrer" target="_blank">http://www.rbvi.ucsf.edu/<wbr>chimera/docs/UsersGuide/midas/<wbr>ksdssp.html</a>><br>
I hope this helps,<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D.<br>
UCSF Chimera(X) team<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<div><div class="h5"><br>
> On Jun 27, 2018, at 8:24 AM, Ahmad Abdelzaher <<a href="mailto:underoath006@gmail.com">underoath006@gmail.com</a>> wrote:<br>
> <br>
> Hello,<br>
> <br>
> After saving the two side by side structures in a single file, I get the weird display errors in the picture shown below. How can I fix that?<br>
> <br>
> Regards. <br>
</div></div>> <before_saving.png><after_<wbr>Saving.png><br>
</blockquote></div><br></div>