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<p class="MsoNormal">Dear, </p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am trying to build a few homology models using the sequence of mouse HSP90 alpha and beta, and I have a few questions:
</p>
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<li class="MsoNormal" style="mso-list:l1 level1 lfo1">First I tried to build a model of mouse Hsp90 using its protein sequence and 2CG9 (a dimer) as a template. The proposed model came out as a monomer, which is understandable since the sequence provided is
of a monomer. However, I am wondering if there is a way for me to build a dimer using this monomer sequence with a dimer template (write it as a dimer sequence somehow? Or there is a specific option to do this?).
<o:p></o:p></li><li class="MsoNormal" style="mso-list:l1 level1 lfo1">I also want to build a homology model using 2IOQ as a template. However, on PDB, 2IOQ is displayed as 3 structures (biological assembly 1, biological assembly 2, asymmetrical structure). The structure I
desire as a template is biological assembly 2 but when I fetched it by ID, the asymmetrical model came out and is also the template the program used. Is there a way for me to specify the template?
<o:p></o:p></li><li class="MsoNormal" style="mso-list:l1 level1 lfo1">I have a protein model of my own that is not on PDB. Is there a way for me to use my structure as a template for the sequence? If yes, how would I do so?
<o:p></o:p></li></ol>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Please let me know as soon as possible. Thank you very much.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Sincerely, </p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Hieu Nguyen <br>
Research Assistant - Resident Assistant<br>
University of New Hampshire '18<br>
Biochemistry, Molecular and Cellular Biology<br>
Department of Molecular, Cellular and Biomedical Sciences </p>
<p class="MsoNormal"><o:p> </o:p></p>
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