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<p class="MsoNormal" style="margin:0in 0in 12pt"><font size="2"><span style="font-family:arial,helvetica,sans-serif">Hi Wil,<span></span></span></font></p><font size="2"><span style="font-family:arial,helvetica,sans-serif">
</span></font><p class="MsoNormal" style="margin:0in 0in 12pt"><font size="2"><span style="font-family:arial,helvetica,sans-serif">Have you
tried ConSurf server? <a href="http://consurf.tau.ac.il">http://consurf.tau.ac.il</a><span></span></span></font></p><font size="2"><span style="font-family:arial,helvetica,sans-serif">
</span></font><p class="MsoNormal" style="margin:0in 0in 0.0001pt"><font size="2"><span style="font-family:arial,helvetica,sans-serif">I haven't tried for a long time,
but I think it can perform a sequence analysis using the sequence associated to
the PDB ID (3V0I for you) and then it will map the residue conservation on to the
structure. One the files in the results page will be a kind of Chimera session
file. You need to download and open it in Chimera, then you’ll see the
highlighted residues according to the conservation score.<span></span></span></font></p><font size="2"><span style="font-family:arial,helvetica,sans-serif">
<br></span></font></div><font size="2"><span style="font-family:arial,helvetica,sans-serif">Regards,</span></font><br><br></div>Aldo<br></div><div class="gmail_extra"><br><div class="gmail_quote">2018-02-12 11:38 GMT+00:00 Wil Ratzan <span dir="ltr"><<a href="mailto:wilratzan@gmail.com" target="_blank">wilratzan@gmail.com</a>></span>:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">Hello,<div><br></div><div>I truly appreciate the development and distribution of software such as Chimera to the scientific community, but alas I am not 'computer-savvy.'</div><div><br></div><div>I would like to map highly conserved residues of a protein onto its crystal structure (PDB 3V0I). I can view the PDB file of the crystal structure using Chimera, but I am having a hard time figuring out how to highlight residues. Could you please give me some advice about how to identify and mark particular residues using this file?</div><div><br></div><div>I apologize if this request seems trivial, and I appreciate any advice.</div><div><br></div><div>Thanks,</div><div><br></div><div>-Wil Ratzan</div><div><br></div><div>Dept. of Cell Biology and Neuroscience</div><div>Montana State University</div><div>Bozeman, MT</div><div>59717</div><div><br></div></div>
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<br></blockquote></div><br><br clear="all"><br>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr">=========================================<br>Aldo Segura-Cabrera<div dir="ltr">Chemogenomics Scientist</div><div dir="ltr">European Bioinformatics Institute (EMBL-EBI)<br>Wellcome Trust Genome Campus <br>Hinxton, Cambridge, UK<span></span><br><div>e-mail: <a href="mailto:asegurac@ipn.mx" target="_blank">asegura@ebi.ac.uk</a>; <a href="mailto:aldosegura@gmail.com" target="_blank">aldosegura@gmail.com</a><br>website: <a href="http://aseguralab.org/" target="_blank">http://aseguralab.org/</a></div></div><div>=========================================</div></div></div></div></div></div></div></div></div></div></div>
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