<div dir="ltr">Hello,<div><br></div><div>I have a protein model that I colored according to region:</div><div><br></div><div>N-terminal domain (green), </div><div>a domain that I inserted (gray),</div><div>and the flanking (GGS)2 linkers (tan). </div><div><br></div><div>When I apply PipesAndPlanks, the tan regions are changed to either green or gray, which is not desired (and apparently, the PipesAndPlanks' color cannot be changed once rendered). Is this a bug or am I doing something wrong?</div><div><br></div><div>To add a little more detail, I manually Frankensteined this model together. At the genetic level, it's just a domain insertion, flanked by linkers, but in Chimera I did this by: </div><div>Opening my wt protein model (green), splitting the chain into two models at an internal site, generating GGSGGS linkers de novo in Chimera and joining these to the new models, and then finally joining those two fragments through a "bridge" of another model. There was lots of manual torsioning and minimizing using FoldIt in this process.</div><div><br></div><div>I can imagine a work around is just to overlay two PipesAndPlanks renders and masking different regions, but this seems inelegant.</div><div><br></div><div>Any assistance/advice is appreciated!</div><div><br></div><div><div>--Andrew</div><div class="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"></div></div></div></div></div></div>
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