<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hello Frances,<div class="">I see Tom also answered, but we came at the answers from some different angles, so I’m sending my reply too in case it adds anything…</div><div class=""><br class=""><div class="">There is no need to do any splitting. You could just open 3iyu and then use Multiscale Models “Make models”. In the result, you can select and color individual chain surfaces. Select with the mouse is Ctrl-click, and there are several buttons in the Multiscale Models dialog “Select chains” section (top part) to control which ones are selected, as indicated with green outlines. The “Act on selected chains” section of Multiscale Models (middle) includes a color well and other options. Clicking the square color well shows the Color Editor, in which you can interactively choose a color for the selected chain(s). That colors a whole chain’s surface one color. As Tom mentioned you can select and color all the symmetry copies the same if you want, or color individual chains different colors.</div><div class=""><br class=""></div><div class="">By default, Multiscale Models shows a low-resolution surface for all the copies that were added to create the overall assembly, and only has atomic detail for the original copy of 3iyu. If you want to see low-res surfaces for all, including the original copy, you would use Select chains “With loaded atoms” (in the Multiscale Models dialog) and then in Act on selected chains, “Show” selected chains.</div><div class=""><br class=""></div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multiscale/framemulti.html" class="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multiscale/framemulti.html</a>></div><div class=""><br class=""></div><div class="">To color individual residues, you need to have atomic detail loaded. The original copy of the structure is already loaded, so if you only wanted to do it in one copy you might as well use that one. Detailed coloring can be done in the ribbon or atomic representations (stick, sphere, etc.); if you want to color the surface by specific atoms/residues, however, you may want to increase the resolution of that surface first. For example, select the chains with loaded atoms as described above, change resolution in the Act on selected chains section to some lower number (default is 8, I tried 3… setting it to 0 would calculate the standard high-resolution molecular surface, but that gives a lot of errors on structure 3iyu)</div><div class=""><br class=""></div><div class="">Next you would color the atoms as desired, for example, command:</div><div class=""><br class=""></div><div class="">color red :100-200.N</div><div class=""><br class=""></div><div class="">… to color residues 100-200 in chain N red. Then select them and use Color Zone, commands:</div><div class=""><br class=""></div><div class="">select :100-200.N</div><div class="">start Color Zone</div><div class=""><br class=""></div><div class="">… click Color button and adjust the zone distance. I have trouble controlling the slider so I just enter a number directly. I attached an image of the result at that point.</div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/colorzone/colorzone.html" class="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/colorzone/colorzone.html</a>></div><div class=""><br class=""></div><div class="">I hope this helps,</div><div class="">Elaine</div><div class=""><div class="">----------<br class="">Elaine C. Meng, Ph.D. <br class="">UCSF Chimera(X) team<br class="">Department of Pharmaceutical Chemistry<br class="">University of California, San Francisco<br class=""><br class=""><img apple-inline="yes" id="7403F9BF-AD83-4E99-AF0D-C25B3E04CA37" src="cid:F2DF36BF-AF78-4125-8894-23B6468BF696@compbio.ucsf.edu" class=""></div><br class=""><blockquote type="cite" class="">On Oct 18, 2017, at 11:00 AM, Frances Shepherd <<a href="mailto:sheph085@umn.edu" class="">sheph085@umn.edu</a>> wrote:<br class=""><br class="">Hello,<br class=""><br class="">I’m working with a rotavirus capsid structure and I’d like to highlight individual residues on it if possible. I’m using PDB #3IYU which I split into individual chains, and used the multiscale model function to create the capsid structure. I’m able to color different surfaces, but it applies the color to each spot where the chain is on the entire capsid, rather than just one subunit. I’d like to recreate the kind of figure from a paper by Zeller et al (2012), attached below. Additionally, is it possible to color individual residues on a capsid structure? <br class=""><br class="">Thanks in advance!<br class="">-Frances Shepherd<br class=""><span id="cid:C393EA3F-3689-4189-9F92-E552E8BCE393@umn.edu"><Screen Shot 2017-10-18 at 12.58.29 PM.png></span><br class=""></blockquote><br class=""></div></div></body></html>