<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Dear Elaine,<div class=""><br class=""></div><div class="">It is me and not Dina who is trying to use Chimera Modeller interface and I am having several problems. I tried to do what you suggested and it does not work. The error message is “could not determine structure to load for selected template” and the modeller does not run the calculation. I am sending you my pdb file and the sequence alignment file. Could you look at it and tell me what is wrong? </div><div class="">Also, I was having trouble with two chains being in the file. I would like to model one chain, but in the presence of the second chain. I do not 100% understand what should be in the comments in the .ali file and if I inserted them correctly:</div><div class=""><br class=""></div><div class="">Here:</div><div class="">structureX:5C7Jcut: 520 :A: 75 :C:::: </div><div class="">sequence:llama: 520 :A: 75 :C::::</div><div class=""><br class=""></div><div class="">I would be very grateful for you if you could solve me these problems.</div><div class=""></div></body></html>