<div dir="ltr"><div><div>Okay thank you Sir.<br></div>I got it. Your guidance was really helpful.<br></div><div>Thank you.<br></div></div><div class="gmail_extra"><br><div class="gmail_quote">On Fri, Oct 7, 2016 at 10:28 PM, Elaine Meng <span dir="ltr"><<a href="mailto:meng@cgl.ucsf.edu" target="_blank">meng@cgl.ucsf.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Dear Shreyas Iyer,<br>
<br>
You can compare related protein structures by superimposing them with MatchMaker (in menu under Tools… Structure Comparison) or its command version “matchmaker”. This will give the RMSD in the Reply Log (menu: Favorites.. Reply Log).<br>
<br>
Description and instructions, graphical interface:<br>
<<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/matchmaker/matchmaker.html" rel="noreferrer" target="_blank">http://www.rbvi.ucsf.edu/<wbr>chimera/docs/<wbr>ContributedSoftware/<wbr>matchmaker/matchmaker.html</a>><br>
<br>
…command, including examples:<br>
<<a href="http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/mmaker.html" rel="noreferrer" target="_blank">http://www.rbvi.ucsf.edu/<wbr>chimera/docs/UsersGuide/midas/<wbr>mmaker.html</a>><br>
<br>
Matchmaker uses only one atom per residue, the alpha-carbons (CA atoms). If you want to use more atoms or different atoms you would have to use the “match” command instead.<br>
<<a href="http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/match.html" rel="noreferrer" target="_blank">http://www.rbvi.ucsf.edu/<wbr>chimera/docs/UsersGuide/midas/<wbr>match.html</a>><br>
<br>
I hope this helps,<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D.<br>
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<br>
P.S. The email address for Chimera questions is <a href="mailto:chimera-users@cgl.ucsf.edu">chimera-users@cgl.ucsf.edu</a> (as CC’d here).<br>
<div class="HOEnZb"><div class="h5"><br>
On Oct 7, 2016, at 4:09 AM, Shreyas Iyer <<a href="mailto:shreyas.i.imsc14@ahduni.edu.in">shreyas.i.imsc14@ahduni.edu.<wbr>in</a>> wrote:<br>
<br>
> Respected Sir/Madam,<br>
><br>
> I wanted to know how to calculate RMSD values between any 2 homology models of a protein with the help of USCF Chimera. Please guide me as soon as possible.<br>
><br>
> Awaiting a positive reply.<br>
><br>
> Yours Sincerely,<br>
> Shreyas Iyer<br>
<br>
</div></div></blockquote></div><br></div>