<html><head></head><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div id="yui_3_16_0_ym19_1_1463169526182_4345"><span id="yui_3_16_0_ym19_1_1463169526182_4346">Hi Elaine,</span></div><div id="yui_3_16_0_ym19_1_1463169526182_4347"><br clear="none" id="yui_3_16_0_ym19_1_1463169526182_4348"></div><div id="yui_3_16_0_ym19_1_1463169526182_4349"><span id="yui_3_16_0_ym19_1_1463169526182_4350">Thank you for the quick response. I was doing my work via windows, not the command line. Is there something I need to do to keep all of the surfaces/sequences open??</span></div><div dir="ltr" id="yui_3_16_0_ym19_1_1463169526182_4351"><span id="yui_3_16_0_ym19_1_1463169526182_4352">-Danny</span></div> <div class="qtdSeparateBR"><br><br></div><div class="yahoo_quoted" style="display: block;"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;"> <div dir="ltr"><font size="2" face="Arial"> On Friday, May 13, 2016 3:57 PM, Elaine Meng <meng@cgl.ucsf.edu> wrote:<br></font></div> <br><br> <div class="y_msg_container"><div id="yiv9361172848"><div>Hi Dan,<div>I wasn’t able to reproduce this problem. I just tested in Chimera 1.10.2 on 3 related proteins, with commands:</div><div><br clear="none"></div><div>open 2mnr</div><div>open 1muc</div><div>open 4enl</div><div>delete :.b</div><div>mm #0 #1</div><div>mm #0 #2</div><div>tile</div><div>surf</div><div><br clear="none"></div><div>… then invoking Coulombic Surface Coloring, just clicking Apply because all three surfaces were already chosen in the dialog. Image of the result attached (after applying publication preset 1). </div><div><br clear="none"></div><div>I can’t think of any reason your system would behave differently, and all I can say is to make sure all the surfaces are chosen in the dialog. However, if that still doesn’t work you can always try submitting a bug report (menu: Help… Report a Bug) and attach the session so we could try to reproduce it.</div><div><br clear="none"></div><div>Best,</div><div>Elaine</div><div class="yiv9361172848yqt9736912235" id="yiv9361172848yqt15819"><div><div>-----<br clear="none">Elaine C. Meng, Ph.D. <br clear="none">UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab<br clear="none">Department of Pharmaceutical Chemistry<br clear="none">University of California, San Francisco<br clear="none"></div><div><br clear="none"></div><div><img id="yiv93611728484690812F-F341-4488-9BC1-F77139797456" height="470" width="480" src="cid:7F084832-4475-4F7F-951E-0309CBE9D0A2@cgl.ucsf.edu"></div>On May 13, 2016, at 11:10 AM, zona cat <<a rel="nofollow" shape="rect" ymailto="mailto:azrucu@yahoo.com" target="_blank" href="mailto:azrucu@yahoo.com">azrucu@yahoo.com</a>> wrote:<br clear="none"><br clear="none"><blockquote type="cite">Good afternoon,<br clear="none"><br clear="none">My name is Daniel Heller, and I am working with Helen Berman at Rutgers University on a PDB project. We are trying to analyze multiple DNA binding proteins simultaneously (via matchmaker) and looking to visualize the surface electrostatic potential of each structure as a tiled picture. We are deleting the nucleotide chains for all proteins when complexed with DNA. However, I am only able to get the surface electrostatic coloring for one structure (the reference structure), subsequent structures will not apply the columbic coloring after i hit apply, they just maintain the monotone default surface structure . Could you please help me with this issue.<br clear="none"><br clear="none">Thank you very much,<br clear="none">Dan<br clear="none"></blockquote><br clear="none"></div></div></div></div><br><br></div> </div> </div> </div></div></body></html>