<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class=""></div><div class=""><br class=""></div><div class=""><div class=""><div><blockquote type="cite" class=""><div class="">On Apr 15, 2016, at 1:16 AM, Michael Sharkey &lt;<a href="mailto:michael.sharkey@ucd.ie" class="">michael.sharkey@ucd.ie</a>&gt; wrote:</div><br class="Apple-interchange-newline"><div class=""><meta http-equiv="Content-Type" content="text/html; charset=utf-8" class=""><div dir="ltr" class="">Thanks Dave<div class=""><br class=""></div><div class="">I had saved a copy containing one of the two chains in the asymmetric unit, not realising that the waters didn't come with the protein (and heteroatoms).</div><div class=""><br class=""></div><div class="">Problem solved</div><div class=""><br class=""></div><div class="">Thanks again (for a very swift response)</div><div class=""><br class=""></div><div class="">Mike</div></div><div class="gmail_extra"><br class=""><div class="gmail_quote">On 6 April 2016 at 16:23, Gae, David Hyon <span dir="ltr" class="">&lt;<a href="mailto:DavidHyon.Gae@ucsf.edu" target="_blank" class="">DavidHyon.Gae@ucsf.edu</a>&gt;</span> wrote:<br class=""><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi Mike,<br class="">
<br class="">
If you haven’t messed around the PDB file in WordPad. i think doing “ disp: hoh “ in command line should show the water.<br class="">
<br class="">
<br class="">
Hope this help,<br class="">
Dave<br class="">
&gt; On Apr 6, 2016, at 7:38 AM, Michael Sharkey &lt;<a href="mailto:michael.sharkey@ucd.ie" class="">michael.sharkey@ucd.ie</a>&gt; wrote:<br class="">
&gt;<br class="">
&gt; Hi<br class="">
&gt;<br class="">
&gt; I'm trying to visualise water molecules associated with the crystal structure of an enzyme (specifically PDB ID: 1C1D).&nbsp; They're listed when I open the pdb as a text file using WordPad, but I can't for the life of me get them to appear on the screen when I try to visualise the structure in Chimera (v. 1.10.2).<br class="">
&gt;<br class="">
&gt; What am I missing?<br class="">
&gt;<br class="">
&gt; Thanks in advance<br class="">
&gt;<br class="">
&gt; Mike<br class="">
<span class="HOEnZb"><font color="#888888" class="">&gt;<br class="">
&gt; --<br class="">
&gt;<br class="">
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</font></span></blockquote></div><br class=""><br clear="all" class=""><div class=""><br class=""></div>-- <br class=""><div class="gmail_signature"><span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class="">Michael Sharkey PhD,</span><br style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class=""><span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class="">UCD School of Biomolecular and Biomedical Science,</span><br style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class=""><span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class="">Conway Institute,</span><br style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class=""><span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)" class="">University College Dublin,</span><div class=""><font color="#222222" face="arial, sans-serif" class="">Tel. 01-7166932<br class=""></font><div class=""><br class=""></div></div></div>
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