<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto"><div>Thanks Elaine.&nbsp;</div><div id="AppleMailSignature"><br></div><div id="AppleMailSignature">I was omitting the .A. Since they only had one chain I didn't think it was required. But it's working now.&nbsp;</div><div id="AppleMailSignature"><br></div><div id="AppleMailSignature">Best,</div><div id="AppleMailSignature"><br></div><div id="AppleMailSignature">Kyle<br><br><p style="font-size: 12px; -webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-composition-fill-color: rgba(175, 192, 227, 0.230469); -webkit-composition-frame-color: rgba(77, 128, 180, 0.230469); -webkit-text-size-adjust: auto; -webkit-text-stroke-width: initial; margin: 0px; font-family: Arial; -webkit-text-stroke-color: rgb(0, 0, 0); "></p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; "></p><p style="font-size: 12px; -webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-composition-fill-color: rgba(175, 192, 227, 0.230469); -webkit-composition-frame-color: rgba(77, 128, 180, 0.230469); -webkit-text-size-adjust: auto; -webkit-text-stroke-width: initial; margin: 0px; font-family: Arial; -webkit-text-stroke-color: rgb(0, 0, 0); "></p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; "><span style="font-family: Arial-BoldMT; font-weight: bold; ">Dr. Kyle J. Lauersen</span></p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; ">Algae Biotechnology &amp; Bioenergy</p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; ">Center for Biotechnology</p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; ">Bielefeld University</p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; ">p: +49 (0) 521 106 12261</p><p style="-webkit-tap-highlight-color: rgba(26, 26, 26, 0.296875); -webkit-text-stroke-width: initial; margin: 0px; ">e: <a href="mailto:kyle.lauersen@uni-bielefeld.de">kyle.lauersen@uni-bielefeld.de</a></p><p></p><p></p><p></p></div><div><br>On 29.01.2016, at 20:43, Elaine Meng &lt;<a href="mailto:meng@cgl.ucsf.edu">meng@cgl.ucsf.edu</a>&gt; wrote:<br><br></div><blockquote type="cite"><div><span>Hi Kyle,</span><br><span>The following works for me:</span><br><span></span><br><span>I opened YFP and PcPs in that order, then:</span><br><span></span><br><span>commands: </span><br><span>~ribbon</span><br><span>disp @n,ca,c</span><br><span>sel #1:559.a@C #0:2.a@N</span><br><span></span><br><span>menu: Tools….Structure Editing… Build Structure, change to Join Models section of the resulting dialog.</span><br><span></span><br><span>Then I chose the “C-N peptide bond” option and clicked Apply, and the join appears to take place successfully.</span><br><span></span><br><span>However, I see your point that something weird is going on: &nbsp;I repeated the process several times, and sometimes (but not always!!) if I didn’t change from ribbon to atom display, the Apply button was grayed out. &nbsp;We should look into why that is happening.</span><br><span></span><br><span>I hope this helps,</span><br><span>Elaine</span><br><span>-----</span><br><span>Elaine C. Meng, Ph.D. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><br><span>UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab</span><br><span>Department of Pharmaceutical Chemistry</span><br><span>University of California, San Francisco</span><br><span></span><br><span></span><br><span></span><br><span>On Jan 29, 2016, at 5:33 AM, Kyle J. Lauersen &lt;<a href="mailto:kyle.lauersen@uni-bielefeld.de">kyle.lauersen@uni-bielefeld.de</a>&gt; wrote:</span><br><span></span><br><blockquote type="cite"><span>Hello,</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>I am trying to join the two models attached to this sequence.</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>I have used the sequence selection code in atom specifier: #1:559.a@C #0:2.a@N</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>Where model #0 is YFP and #1 is PcPs.</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>When I go to join models, the Apply button is not accessible.</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>Can you indicate if I am doing something incorrect in the selection of the C and N atoms?</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>Thank you for your time.</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>Kyle</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>&lt;YFP.pdb&gt;</span><br></blockquote><blockquote type="cite"><span>&lt;PcPs.pdb&gt;</span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>Dr. Kyle J. Lauersen</span><br></blockquote><blockquote type="cite"><span>Algae Biotechnology &amp; Bioenergy </span><br></blockquote><blockquote type="cite"><span>Center for Biotechnology </span><br></blockquote><blockquote type="cite"><span>Bielefeld University </span><br></blockquote><blockquote type="cite"><span>p: +49 (0) 521 106 12289</span><br></blockquote><blockquote type="cite"><span>e: <a href="mailto:kyle.lauersen@uni-bielefeld.de">kyle.lauersen@uni-bielefeld.de</a></span><br></blockquote><blockquote type="cite"><span></span><br></blockquote><blockquote type="cite"><span>_______________________________________________</span><br></blockquote><blockquote type="cite"><span>Chimera-users mailing list</span><br></blockquote><blockquote type="cite"><span><a href="mailto:Chimera-users@cgl.ucsf.edu">Chimera-users@cgl.ucsf.edu</a></span><br></blockquote><blockquote type="cite"><span><a href="http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users">http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users</a></span><br></blockquote><span></span><br></div></blockquote></body></html>