<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class="">Hi Zhihai,</div><div class=""><br class=""></div>Yes use molmap then vop resample. If your atomic model is #0 and experimental map is #1 with resolution 5 Angstroms use<div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>molmap #0 5</div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>vop resample #2 ongrid #1</div><div class=""><br class=""></div><div class="">For computing FSC it should not matter what resolution you use with molmap because that resolution value will just scale the Fourier shells and should not alter the correlation with a shell. But I would test to make sure this is the case. Also if you are at very high resolution (3 - 4 Angstroms) I am not sure the molmap map is good enough an approximation since it simply puts a Gaussian at each atom position with all Gaussians having the same standard deviation.</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Jan 22, 2016, at 5:25 AM, 李智海 < wrote:</div><br class="Apple-interchange-newline"><div class=""><meta http-equiv="Content-Type" content="text/html charset=utf-8" class=""><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Maybe now I find the answer to my own question, the command combinations <i class="">molmap onGrid</i> and <i class="">vop resample</i> can solve my problem. <div class=""><br class=""></div><div class="">Zhihai </div><div class=""><div class=""><blockquote type="cite" class=""><div class="">On Jan 22, 2016, at 9:00 PM, 李智海 wrote:</div><br class="Apple-interchange-newline"><div class=""><meta http-equiv="Content-Type" content="text/html charset=us-ascii" class=""><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi,<div class=""><br class=""></div><div class="">Here I want to calculate a model-to-map FSC curve. If I am not wrong, firstly I am supposed to convert the pdb file to a density map. So I was just wondering in Chimera, how to use command <i class="">molmap</i> to generated a map with the same pixel size and dimensions as the corresponding map?</div><div class=""><br class=""></div><div class="">Any help will be high appreciated.</div><div class=""><br class=""></div><div class="">Best,</div><div class=""><br class=""></div><div class="">Zhihai Li</div><div class=""><br class=""></div></div></div></blockquote></div><br class=""></div></div>_______________________________________________<br class="">Chimera-users mailing list<br class=""><a href="mailto:Chimera-users@cgl.ucsf.edu" class="">Chimera-users@cgl.ucsf.edu</a><br class="">http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users<br class=""></div></blockquote></div><br class=""></div></body></html>