<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=us-ascii"><meta name=Generator content="Microsoft Word 14 (filtered medium)"><style><!--
/* Font Definitions */
@font-face
        {font-family:Calibri;
        panose-1:2 15 5 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
        {margin:0cm;
        margin-bottom:.0001pt;
        font-size:11.0pt;
        font-family:"Calibri","sans-serif";
        mso-fareast-language:EN-US;}
a:link, span.MsoHyperlink
        {mso-style-priority:99;
        color:blue;
        text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
        {mso-style-priority:99;
        color:purple;
        text-decoration:underline;}
p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
        {mso-style-priority:34;
        margin-top:0cm;
        margin-right:0cm;
        margin-bottom:0cm;
        margin-left:36.0pt;
        margin-bottom:.0001pt;
        font-size:11.0pt;
        font-family:"Calibri","sans-serif";
        mso-fareast-language:EN-US;}
span.E-MailFormatvorlage17
        {mso-style-type:personal-compose;
        font-family:"Calibri","sans-serif";
        color:windowtext;}
.MsoChpDefault
        {mso-style-type:export-only;
        font-family:"Calibri","sans-serif";
        mso-fareast-language:EN-US;}
@page WordSection1
        {size:612.0pt 792.0pt;
        margin:70.85pt 70.85pt 2.0cm 70.85pt;}
div.WordSection1
        {page:WordSection1;}
/* List Definitions */
@list l0
        {mso-list-id:1978030030;
        mso-list-type:hybrid;
        mso-list-template-ids:-1712559744 67567631 67567641 67567643 67567631 67567641 67567643 67567631 67567641 67567643;}
@list l0:level1
        {mso-level-tab-stop:none;
        mso-level-number-position:left;
        text-indent:-18.0pt;}
@list l0:level2
        {mso-level-number-format:alpha-lower;
        mso-level-tab-stop:none;
        mso-level-number-position:left;
        text-indent:-18.0pt;}
@list l0:level3
        {mso-level-number-format:roman-lower;
        mso-level-tab-stop:none;
        mso-level-number-position:right;
        text-indent:-9.0pt;}
@list l0:level4
        {mso-level-tab-stop:none;
        mso-level-number-position:left;
        text-indent:-18.0pt;}
@list l0:level5
        {mso-level-number-format:alpha-lower;
        mso-level-tab-stop:none;
        mso-level-number-position:left;
        text-indent:-18.0pt;}
@list l0:level6
        {mso-level-number-format:roman-lower;
        mso-level-tab-stop:none;
        mso-level-number-position:right;
        text-indent:-9.0pt;}
@list l0:level7
        {mso-level-tab-stop:none;
        mso-level-number-position:left;
        text-indent:-18.0pt;}
@list l0:level8
        {mso-level-number-format:alpha-lower;
        mso-level-tab-stop:none;
        mso-level-number-position:left;
        text-indent:-18.0pt;}
@list l0:level9
        {mso-level-number-format:roman-lower;
        mso-level-tab-stop:none;
        mso-level-number-position:right;
        text-indent:-9.0pt;}
ol
        {margin-bottom:0cm;}
ul
        {margin-bottom:0cm;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]--></head><body lang=DE link=blue vlink=purple><div class=WordSection1><p class=MsoNormal>Dear Colleagues,<o:p></o:p></p><p class=MsoNormal><o:p>&nbsp;</o:p></p><p class=MsoNormal><o:p>&nbsp;</o:p></p><p class=MsoNormal><span lang=EN-US>Thanks for developing such wonderful tools as Chimera for molecular dynamics analysis of structure.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>Please I have two questions:<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span lang=EN-US><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><![endif]><span lang=EN-US>Is it possible to calculate Gibbs free energy&nbsp; from complexes on UCSF Chimera, &nbsp;for examples when &nbsp;complexes are solvated?<o:p></o:p></span></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span lang=EN-US><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><![endif]><span lang=EN-US>On building structures , is it possible to simulate how a given complex could bind DNA oligos , for example &nbsp;when one makes &nbsp;changes in single nucleotides and ask&nbsp; a given &nbsp;DNA binding TF structure to bind it on Chimera?<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US>I would look forward to your response to these questions. I would also be very happy if I could speak by phone with the administrators.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US>Thanks.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US> <o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>Best wishes,<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p>&nbsp;</o:p></span></p><p class=MsoNormal><span lang=EN-US>Chidiebere U Awah MD Msc<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>PhD student , Molecular Medicine<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>Hannover Medical School , Germany.<o:p></o:p></span></p></div></body></html>