<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi Leila,<div>Here’s an image of your structure and dx map in Chimera, with status line showing the 4 values. The ESP value is given as “nan”, meaning there is nothing useful in the map file because it was calculated without charges.</div><div>Elaine<br><div><br></div><div><img height="537" width="574" apple-width="yes" apple-height="yes" apple-inline="yes" id="1001997D-3F5A-40EC-8728-6665AAAB2FD3" src="cid:1377776B-DDE5-4065-BAB3-3C5A6C611EBF@cgl.ucsf.edu"></div><div><br><div><br></div><br>On Jul 17, 2015, at 1:11 PM, leila karami <<a href="mailto:karami.leila1@gmail.com">karami.leila1@gmail.com</a>> wrote:<br><br><blockquote type="cite">Dear Elaine,<br><br>1) "report values" gives only 3 values (x coord, y coord, z coord) and not 4 values.<br><br>2) Based on your suggestion, I did following steps:<br><br>Tools... structure Editing... pdb2pqr<br><br>But I encountered with:<br><br>Service 'opal:pdb2pqr_1.8' is unavailable.<br><br>3) Again, I tried the apbs in command line.<br><br>To have my dx and the PDB files, please check the following links:<br><br><a href="https://www.dropbox.com/s/n4zv0lq7s4p1pz2/44.pdb?dl=0">https://www.dropbox.com/s/n4zv0lq7s4p1pz2/44.pdb?dl=0</a><br>https://www.dropbox.com/s/w6dg4adb498178r/tmpiwv0lf.dx?dl=0<br><br>Again, electrostatic surface is as black and white instead of red and blue.<br><br>Thanks in advance,<br>Leila<br>_______________________________________________<br>Chimera-users mailing list<br>Chimera-users@cgl.ucsf.edu<br>http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users<br></blockquote><br></div></div></body></html>