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<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">I am pleased to inform you that I have been using aspects of Chimera that permit me to prepare images of proteins deposited in the pdb.</FONT></SPAN><SPAN LANG="en-ca"><I> <FONT FACE="Calibri"></FONT></I></SPAN><SPAN LANG="en-ca"> <FONT FACE="Calibri">It was a pleasant surprise to find that the learning curve was not as steep as I thought it might be. Mind you, there are many aspects of Chimera that I have not yet attempted to use.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">Now I wish to do something that may not be possible to do with Chimera. And I am not sure where to look in the tutorials.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">I have a ribbon diagram of a protein-RNA complex, PDB 4p3e, two proteins, SRP 19 and SRP68, bound to a segment of 7SL RNA.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">I can also obtain the structure of another protein SRP54 which also bind to this complex. However, the structure of pdb 4p3e with SRP54 is not available.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">I know where SRP54 should bind to the RNA, and I know which domain of SRP54 binds to the RNA.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">How can I somehow combine the two files, one for SRP54 and the other 4p3e and locate SRP54 onto the 4p3e structure? (not sure it is possible) Of course crystallographers know how to achieve this structural combination. I don’t have a clue.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">I checked the tutorial list and the closest I seemed to come was the Merge command. However, the tutorial did not seem to deal with what I wanted to do.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">Thank you in advance for your assistance.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">Arthur G. Szabo Ph.D.</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">Professor Emeritus, Chemistry</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">Wilfrid Laurier University</FONT></SPAN></P>
<P DIR=LTR><SPAN LANG="en-ca"><FONT FACE="Calibri">Waterloo, Canada.</FONT></SPAN></P>
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