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</o:shapelayout></xml><![endif]--></head><body lang=ES link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span lang=EN-US>Hi,<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US>I am new to Chimera, so perhaps someone could give me a hand. <o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>I have used Robetta for predicting the structure of a protein I am interested in and it gave back models for different parts of the protein. I would like to join them, so I am trying to join two models by a C-N peptide bond. I have selected the corresponding atoms in the last amino acid in one of the proteins and in the first amino acid of the other protein I want to join. With both atoms selected I took the “Join Models” option in “Build Structure” with the “C-N peptide bond” selected. And the “Apply” button does not activate. (See the screen shot attached)<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US>I have gone through the manual and I do not see what I am missing or what I am doing wrong. Any help is welcome.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US>Thank you very much in advance.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>Kind regards,<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-GB style='color:#1F497D'>Miguel</span><i><span lang=EN-GB style='font-size:8.0pt;font-family:"Arial","sans-serif";color:green'><o:p></o:p></span></i></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p></div></body></html>