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<p>I have recently been getting some imod segmentation files and have been noticing three problems<br>
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<p>1) when I read the MRC and imod files I am having a scaling problem, and the datasets do not integrate.<br>
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<p>For example, if the MRC voxel size is 31.22 31.22 31.22, and the origin index is 0 -114 0, I have to change the the voxel size to
<span style="font-size: 12pt;">3.122 </span>3.122 3.122 , and the origin index to <span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">0 0 0 so that graphics match correctly. Since there are
number of people in the data chain before it gets to me, I thought it might check to determine if this is known problem before going upstream to unknown people to figure out if they are getting the imod output settings correct.</span><br>
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<p><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">2) The graphics performance with imod segmentation files is sluggish in chimera, not sure what it is in imod. I assume that the format
defines the vertices at the highest resolution corresponding with the MRC file. Is there a way to improve the performance on the chimera side? I am not using contouring, just reading the file in default mode.</span></p>
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<p><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">3) The segmentation has a lot of components, such as vesicles and microtubules, that have different colors. Is there a way to break
apart the components so they can be manipulated seperately? E.g., display/hide, change colors, translate and rotate.</span></p>
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<div class="PlainText">Matthew Dougherty<br>
National Center for Macromolecular Imaging<br>
Baylor College of Medicine<br>
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