<html><body><div style="color:#000; background-color:#fff; font-family:HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif;font-size:16px"><div class="" style=""><span class="" style="">Thank you Elaine. I appreciate it. </span></div><div class="" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><span class="" style=""><br></span></div><div class="" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><span class="" style="">I have been able to acetylate the lysine residues but now I am having difficulty in eliminating the charge. </span>I noticed that each hydrogen on a normal epsilon amino group of lysine is assigned +0.34 charge. It
would obviously be less than +1 and when I try and assign it +0 it basically is not capable of conducting the calculation. I will continue to read and work on it.</div><div class="" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><span style="background-color: transparent;"><br></span></div><div class="" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><span style="background-color: transparent;">Thanks,</span></div><div class="" style="color: rgb(0, 0, 0); font-size: 16px; font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; background-color: transparent; font-style: normal;"><span style="background-color: transparent;">Jesse
</span><br></div> <div class="qtdSeparateBR"><br><br></div><div class="yahoo_quoted" style="display: block;"> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" class=""> <div style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;" class=""> <div dir="ltr" class="" style=""> <font size="2" face="Arial" class="" style=""> On Tuesday, October 21, 2014 1:18 PM, Elaine Meng <meng@cgl.ucsf.edu> wrote:<br class="" style=""> </font> </div> <br class="" style=""><br class="" style=""> <div class="" style="">I should have added an "etc." … the steps I mentioned still wouldn't be the whole acetyl group!<br class="" style="">Elaine<br class="" style=""><br class="" style=""><br class="" style="">On Oct 21, 2014, at 11:06 AM, Elaine Meng <<a ymailto="mailto:meng@cgl.ucsf.edu" href="mailto:meng@cgl.ucsf.edu" class=""
style="">meng@cgl.ucsf.edu</a>> wrote:<br class="" style=""><br class="" style="">> Hi Jesse,<br class="" style="">> You can manually build onto your structure atom-by-atom using Build Structure (in menu under Tools… Structure Editing). It's not the most friendly builder, but once you play around with it for a while, you can see how the general process works. Basically to build outward you use the "Modify Structure" tab, with cycles of hydrogen addition and then changing a hydrogen to some other atom type. You would add hydrogens (could do with AddH the first time around… this tool is also in the Structure Editing section), then change one hydrogen to a carbon of the proper hybridization type, then add hydrogens again, then change one of the hydrogens on the carbon to an oxygen of the proper hybridization type.<br class="" style="">> <br class="" style="">> See the following (or click Help on the tool dialog)<br
class="" style="">> <<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.html" target="_blank" class="" style="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.html</a>><br class="" style="">> <br class="" style="">> Best,<br class="" style="">> Elaine<br class="" style="">> ----------<br class="" style="">> Elaine C. Meng, Ph.D. <br class="" style="">> UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab<br class="" style="">> Department of Pharmaceutical Chemistry<br class="" style="">> University of California, San Francisco<br class="" style="">> <br class="" style="">> On Oct 21, 2014, at 10:55 AM, JESSE JAYNES <<a ymailto="mailto:jjsqrd@bellsouth.net" href="mailto:jjsqrd@bellsouth.net" class="" style="">jjsqrd@bellsouth.net</a>> wrote:<br class="" style="">> <br class="" style="">>> Folks:<br class="" style="">>> I was
wondering if I could get help with something. I have a peptide with a peptide with lysines and I would like to add acetyl groups to them. Is that possible? Thanks, Jesse<br class="" style="">> <br class="" style=""><br class="" style=""><br class="" style=""></div> </div> </div> </div> </div></body></html>