<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi Christian,<div><br></div><div> Set the “voxel size” in the Coordinates panel of the Chimera Volume Viewer dialog (menu Features / Coordinates) to 8.6. The Tomography Toolbox format *.em does include a voxel size value and Chimera reads it but apparently it is not set or it is set to 1 Angstrom in your map file. If you save your map as say MRC it will have the new voxel size. Or if you save a Chimera session it will have the new voxel size. Chimera does not write Tomography Toolbox format so it cannot rewrite that file.</div><div><br></div><div><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div><br></div><div><br><div><div>On May 13, 2014, at 2:06 AM, Christian Geiss wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
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Dear Chimera User list,<br>
<br>
I have a fundamental problem when loading pdb structures and EM
density maps as *.em. The scale doesn't fit at all, with other words
my pdb structure<br>
is tremendously bigger than the EM structure. One work-around is to
resample the EM structure from current 64 px boxsize with 8.6 A
pixelsize to 1 A pixelsize, giving<br>
~ a 500 pixel volume, but then the volume gets quite big und my
chimera gets really slow in handling those structures (pdb and big
EM map at once)...is there any other way to solve this issue like
scaling the EM structure on the fly in not such a memory consuming
way...I also wonder if there is any information about the pixelsize
in the EM header, because then the scaling should be done usually
automatically???<br>
<br>
Thanks in advance,<br>
<br>
Chris<br>
<br>
P.S. I couldn't find anything related to this in the chimera
archive...<br>
<div class="moz-signature">-- <br>
<b>Christian Geiss, M.sc.</b><br>
Universitaet Frankfurt BMLS<br>
Frangakis Group<br>
Max-von-Laue-Strasse 15<br>
D - 60438 Frankfurt am Main<br>
Phone: +49-(0)69-79842597<br>
Fax: +49-(0)69-79846467<br><br>
</div>
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