<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><div>Hi Francesco,</div><div><span class="Apple-tab-span" style="white-space:pre"> </span>Well, another possibility is to open open PDB files with just ACE/NME and use the "Join Models" tab of Build Structure to connect them to the N/C terminus of your chain. Select the N/C of your chain and the C/N of the ACE/NME and use the "Form C-N peptide bond" option of the Join Models tab and click Apply. If the ACE/NME didn't have the right residue number you might then need to use the Renumber Residues tool to get the numbering you want.</div><div><span class="Apple-tab-span" style="white-space:pre"> </span>Also, unless you use the build that come out tomorrow you may get an error message as you select the N in NME. It's a harmless error that you can ignore.</div><div><span class="Apple-tab-span" style="white-space:pre"> </span>I've attached PDB files of isolated ACE and NME residues for your convenience.</div><div><br></div><div>--Eric</div><div><br> Eric Pettersen<br> UCSF Computer Graphics Lab<br> <a href="http://www.cgl.ucsf.edu">http://www.cgl.ucsf.edu</a><br><br></div><div></div></div></body></html>