<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Dear Elaine Meng,<div><br></div><div>I tryed the surface option in different pdb files (also the 50S of E.coli) and always the same error (code 11- see attached). I also tried the different option of the webpage (Surface calculation failures and workaround).</div><div>I would like to have the surface structure of the chain 1 (25S of the yeast ribosome). Now I use the pdb file 3U5E and 3U5D. Please can you help me. Chimera is such a beautiful program and it always looks good, but in the most publication the surface structure is built up. </div><div><br></div><div>Thank you</div><div><br></div><div>Best regards</div><div><br></div><div>Julian <br><div><div><br></div><div><br></div><div><br></div><div><br></div><div><img apple-inline="yes" id="0FAB2B27-BF0F-43E5-9D93-600924738CDF" height="169" width="320" apple-width="yes" apple-height="yes" src="cid:CC0FAD76-2F74-4C04-A148-33E5834DC781@rz.uni-frankfurt.de"></div><div>Am 06.03.2014 um 19:20 schrieb Elaine Meng <<a href="mailto:meng@cgl.ucsf.edu">meng@cgl.ucsf.edu</a>>:</div><br class="Apple-interchange-newline"><blockquote type="cite">Dear Julian,<br>The surface failure is a numerical thing that may differ on different machines, and there is not a consistent recipe for how to solve the problem. Please see this page on possible solutions, including showing some alternative types of surface instead of a "molecular" surface:<br><br><<a href="http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/surfprobs.html">http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/surfprobs.html</a>><br><br>I hope this helps,<br>Elaine<br>----------<br>Elaine C. Meng, Ph.D. <br>UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab<br>Department of Pharmaceutical Chemistry<br>University of California, San Francisco<br><br>On Mar 4, 2014, at 3:31 AM, Julian Marzi <<a href="mailto:julian.marzi@googlemail.com">julian.marzi@googlemail.com</a>> wrote:<br><br><blockquote type="cite">Dear Chimera,<br>I try to show the surface of the 60S in yeast (PDB: 3O5H), but it doesn't work. Mscalc returned code 11 is shown. If I split the 60S every chain show the surface except the first strain (25S). How can I show the surface from the first chain?<br>Thank you very much<br>Best regards<br>Julian Marzi<br></blockquote><br></blockquote></div><br></div></body></html>