<div style="line-height:1.7;color:#000000;font-size:14px;font-family:arial">Dear professor&nbsp;<span style="white-space: pre-wrap; line-height: 1.7;">Elaine&nbsp;Meng:</span><br>&nbsp; &nbsp;I can not add a GGGGGGG sequence to the place between 182 and 183 residues by the&nbsp;<span style="white-space: pre-wrap; line-height: 1.7;">Modeller&nbsp;interface&nbsp;for&nbsp;building&nbsp;loops. My structure is in a attachment file. Thanks for your help.&nbsp;</span><div><span style="white-space: pre-wrap;"><br></span></div><div><span style="white-space: pre-wrap;"><br></span></div><div><span style="white-space: pre-wrap;">best wishes</span></div><div><span style="white-space: pre-wrap;">Haiping zhang<br></span><br><br><br><div></div><div id="divNeteaseMailCard"></div><br><pre><br>在&nbsp;2013-09-27&nbsp;04:16:42,"Elaine&nbsp;Meng"&nbsp;&lt;meng@cgl.ucsf.edu&gt;&nbsp;写道:
&gt;Dear&nbsp;Haiping&nbsp;Zhang,
&gt;You&nbsp;can&nbsp;use&nbsp;the&nbsp;Modeller&nbsp;interface&nbsp;for&nbsp;building&nbsp;loops.&nbsp;&nbsp;First&nbsp;show&nbsp;the&nbsp;Sequence&nbsp;for&nbsp;that&nbsp;protein&nbsp;chain&nbsp;(menu:&nbsp;Tools…&nbsp;Sequence…&nbsp;Sequence).&nbsp;&nbsp;This&nbsp;sequence&nbsp;comes&nbsp;from&nbsp;the&nbsp;SEQRES&nbsp;in&nbsp;the&nbsp;PDB&nbsp;file&nbsp;and&nbsp;includes&nbsp;the&nbsp;whole&nbsp;sequence.&nbsp;&nbsp;The&nbsp;part&nbsp;that&nbsp;is&nbsp;missing&nbsp;from&nbsp;the&nbsp;structure&nbsp;is&nbsp;shown&nbsp;with&nbsp;a&nbsp;red&nbsp;outline&nbsp;box.&nbsp;&nbsp;To&nbsp;build&nbsp;the&nbsp;missing&nbsp;part,&nbsp;from&nbsp;the&nbsp;Sequence&nbsp;window&nbsp;menu&nbsp;choose&nbsp;Structure…&nbsp;Modeller&nbsp;(loops/refinement).&nbsp;&nbsp;The&nbsp;Modeller&nbsp;loop-building&nbsp;interface&nbsp;is&nbsp;described&nbsp;here:
&gt;
&gt;&lt;http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/modeller.html#building&gt;
&gt;
&gt;I&nbsp;hope&nbsp;this&nbsp;helps,
&gt;Elaine
&gt;----------
&gt;Elaine&nbsp;C.&nbsp;Meng,&nbsp;Ph.D.&nbsp;
&gt;UCSF&nbsp;Computer&nbsp;Graphics&nbsp;Lab&nbsp;(Chimera&nbsp;team)&nbsp;and&nbsp;Babbitt&nbsp;Lab
&gt;Department&nbsp;of&nbsp;Pharmaceutical&nbsp;Chemistry
&gt;University&nbsp;of&nbsp;California,&nbsp;San&nbsp;Francisco
&gt;
&gt;
&gt;On&nbsp;Sep&nbsp;26,&nbsp;2013,&nbsp;at&nbsp;2:59&nbsp;AM,&nbsp;张海平&nbsp;&lt;spr8249618@163.com&gt;&nbsp;wrote:
&gt;
&gt;&gt;&nbsp;Dear&nbsp;professor&nbsp;Elaine&nbsp;C.&nbsp;Meng:
&gt;&gt;&nbsp;&nbsp;&nbsp;&nbsp;I&nbsp;have&nbsp;a&nbsp;structure&nbsp;that&nbsp;miss&nbsp;some&nbsp;resides,&nbsp;I&nbsp;want&nbsp;to&nbsp;add&nbsp;a&nbsp;loop&nbsp;to&nbsp;fed&nbsp;the&nbsp;gap.&nbsp;How&nbsp;can&nbsp;I&nbsp;accomplish&nbsp;this&nbsp;by&nbsp;chimera?&nbsp;The&nbsp;structure&nbsp;is&nbsp;as&nbsp;bellow,&nbsp;I&nbsp;want&nbsp;to&nbsp;add&nbsp;some&nbsp;residus&nbsp;to&nbsp;the&nbsp;place&nbsp;where&nbsp;shows&nbsp;dot&nbsp;line.&nbsp;Thanks&nbsp;for&nbsp;your&nbsp;help.
&gt;&gt;&nbsp;
&gt;&gt;&nbsp;&lt;截图1.png&gt;
&gt;
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