<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:14pt"><div id="yiv4298566528"><div><div style="color: rgb(0, 0, 0); background-color: rgb(255, 255, 255); font-family: 'times new roman', 'new york', times, serif; font-size: 14pt; "><div id="yiv4298566528yui_3_7_2_39_1373612239502_54"><span id="yiv4298566528yui_3_7_2_39_1373612239502_81"><div id="yiv4298566528yui_3_7_2_39_1373612239502_54">Dear All,</div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54"><br></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54">I need to fit atomic structures of two different proteins of different sizes in a density map. The location of both the proteins in the map is well known. While using the fitting tool, I can get the atomic structure of the bigger protein docked very well, the problem arises while fitting the smaller one. The fit option fails and docks the structure into the region
of the density map where the atomic structure of the <span style="font-size:14pt;">larger</span><span style="font-size:14pt;" id="yiv4298566528yui_3_7_2_39_1373612239502_92"> protein </span><span style="font-size:14pt;">is
docked , an incorrect position. </span></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54"><br></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54">How can I fit both the atomic structures in the density map correctly? The resolution of the density map is ~10Å at FSC cutoff of 0.5</div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54"><br></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54">Thanks</div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54"><br></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_54">Andy</div></span></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_56"></div><div id="yiv4298566528yui_3_7_2_39_1373612239502_58"> </div><div id="yiv4298566528yui_3_7_2_39_1373612239502_60"><font class="yiv4298566528Apple-style-span" color="#0000FF" face="garamond, 'new york', times, serif" size="7"><span class="yiv4298566528Apple-style-span" style="font-size:28px;line-height:19px;"><b><blockquote
type="cite" style="color: rgb(0, 0, 0); font-family: Helvetica; font-weight: normal; line-height: normal; font-size: 16px; " class="yiv4298566528yui_3_7_2_39_1373612239502_65"><div><div><div><span class="yiv4298566528Apple-style-span yiv4298566528yui_3_7_2_39_1373612239502_66" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; font-size: 16px; "><span class="yiv4298566528Apple-style-span yiv4298566528yui_3_7_2_39_1373612239502_67" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px;
font-size: 16px; "><div style="word-wrap:break-word;"><div><div><font class="yiv4298566528Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4"><b>Dr. Anindito Sen (Ph.D)</b></font></div><div><font class="yiv4298566528Apple-style-span" color="#341dbb" face="'Times
New Roman'" size="4"><b>Department of Cell Biology & Anatomy</b></font></div><div><font class="yiv4298566528Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4"><b>Graduate School of Medicine</b></font></div><div><font class="yiv4298566528Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4"><b>University of Tokyo</b></font></div><div><font class="yiv4298566528Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4"><b>Tel & fax: +81-3-5841-3339</b></font></div></div></div></span></span></div></div></div></blockquote><div><div><div><div><span class="yiv4298566528Apple-style-span yiv4298566528yui_3_7_2_39_1373612239502_74" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px;
font-size: 16px; "><span class="yiv4298566528Apple-style-span yiv4298566528yui_3_7_2_39_1373612239502_75" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; font-size: 16px; "><div style="word-wrap:break-word;"><div><div><font class="yiv4298566528Apple-style-span" color="#341dbb" face="'Times New Roman'" size="4"><b><br></b></font></div></div></div></span></span></div></div></div></div></b></span></font></div></div></div></div></div></body></html>