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<div class="moz-cite-prefix">You might want to try saving the two
domains as separate PDB's if there are export issues with hidden
domains. If you hide a domain and then go to save pdb and select
"save displayed only" you'll be able to save each domain in it's
own file. As far as file formats go, have you tried VRML? I know
that's pretty universal for 3D printers and might help with
studying the structure as you'd be able to color the atoms by
their atom types. Is your 3D printer capable of color? Another
thing you might want to try is displaying the protein backbone as
ribbon and the sidechain as wire and setting the wire thickness
to a higher number. That might give you the internal detail you
want without saving the data for every single atom. Also,
displaying as Ball and Stick might work too. Those controls are in
the Actions > Atoms/bonds menu. Also, are you wanting to
include the solvent or are you deleting it?
<pre class="moz-signature" cols="72">R.J. Swett
Wayne State University
357 Chemistry
Detroit, MI 48201
Lab Phone 313-577-0552
Cell Phone 906-235-0768</pre>
On 5/19/2013 9:23 PM, Bart Janssen wrote:<br>
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<p class="MsoNormal">Hi<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">My apologies for what are likely to be
stupid questions. We are plant developmental biologists and we
just obtained our first X-ray crystal structure for a new
hormone receptor in plants. We’d really like to make a 3D
model of the protein using one of the 3D printers at our
institute.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I’ve been trying to use and understand
Chimera for the last week and I’m a bit out of my depth :). I
can import our protein 4DNP.pdb and display it using solid
surface or using the sphere representations of atoms. I can
also display it with the “lid” domain hidden and vice versa.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">My problem has been getting files that our
3D printer can read. The solid surface model of the whole
protein can be exported as a scene in .stl format and that can
be printed just fine. Although I can’t figure out how to
adjust resolution or the smoothness of the exported file. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">But I’d really like to print the two
domains of the protein separately. If I use Solid surface
model with the lid hidden what I get is an eggshell like model
which has no internal detail. If I use atoms shown as spheres
instead I get the internal detail I want BUT when I try and
export the scene I get a 2 Gb+ file that is unreadable by the
printer. If I export .obj format instead the colleague who
runs the printer says the file won’t load and appears to have
errors.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have a horrible feeling I am going about
this entirely the wrong way but I can’t find a tutorial that
describes what I’m trying to achieve. I do realise chimera is
not really written for the somewhat trivial use that I am
attempting but it really would be helpful if we had a physical
model of the protein to look at.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Is there a tutorial I should follow or
another part of Chimera (or another program) that I should be
using instead? Any advice would be much appreciated.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Cheers<o:p></o:p></p>
<p class="MsoNormal">Bart<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Bart Janssen<o:p></o:p></p>
<p class="MsoNormal">Scientist<o:p></o:p></p>
<p class="MsoNormal">Plant Development team<o:p></o:p></p>
<p class="MsoNormal">Breeding and genomics<o:p></o:p></p>
<p class="MsoNormal">Plant and Food Research<o:p></o:p></p>
<p class="MsoNormal">Private Bag 92169<o:p></o:p></p>
<p class="MsoNormal">Auckland 1142<o:p></o:p></p>
<p class="MsoNormal">New Zealand<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Ph +64 09 9257179<o:p></o:p></p>
<p class="MsoNormal"><a class="moz-txt-link-abbreviated" href="mailto:bart.janssen@plantandfood.co.nz">bart.janssen@plantandfood.co.nz</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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