<html>
<head>
<meta content="text/html; charset=ISO-8859-1"
http-equiv="Content-Type">
</head>
<body text="#000000" bgcolor="#FFFFFF">
Hi Yuhong,<br>
<br>
I don't know what kind of map you have. If it is a
single-particle electron microscope reconstruction, then the most
common way to determine resolution is Fourier shell correlation.
The original 2-d micrographs are divided into two sets and two 3-d
maps are computed and then you see how far out in frequency the maps
agree. Chimera does not do 3-d reconstructions so this would have
to be done before analysis in Chimera. In the Chimera Fit in Map
dialog it will suggest a default resolution under Options "Use
simulated map from atoms at resolution...". The default is 3 times
the grid plane spacing. Often the map grid spacing is chosen to be
about 1/3 the resolution -- a level of oversampling that provides
nicer visualization.<br>
<br>
I don't understand your origin question. Maybe you mean how do
you find the center of symmetry of a symmetric map. The measure
symmetry command can do that. For example, "measure symmetry #0" if
your map is model #0. Here is documentation.<br>
<br>
<a class="moz-txt-link-freetext" href="http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/midas/measure.html#symmetry">http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/midas/measure.html#symmetry</a><br>
<br>
Tom<br>
<br>
<blockquote
cite="mid:1335928502.72544.YahooMailNeo@web15801.mail.cnb.yahoo.com"
type="cite">
<div style="color:#000; background-color:#fff; font-family:times
new roman, new york, times, serif;font-size:12pt">
<div>Hi friends,</div>
<div>Firstly, I was wondering how to know the resolution of my
map since one day I opened volume viewer...tools...fit to
segments. And I found that there displaying "density map
resolution". So my question is whether that number is the
correct resolution of my map?</div>
<div>Secondly, in the volume viewer...corrdinates I can know the
origin index, but how can I know the origin?</div>
<div>These questions may seem to be stupid, but I just want to
know. So h<span style="font-size: 12pt; ">ope to hear from you
very soon!</span></div>
<div>Best Wishes,</div>
<div>Yuhong Lee</div>
</div>
<br>
</blockquote>
<br>
</body>
</html>