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Hi Brian,<br>
<br>
Yes, if you want to make a density map for a VP7 trimer of
rotavirus and you had 3 monomer VP7 atomic models opened and
positioned to make a trimer in Chimera you would make the map with
Chimera command:<br>
<br>
molmap #0-2 8<br>
<br>
where the monomer model numbers are 0, 1, 2 in this example and the
desired map resolution is 8 Angstroms. As Elaine pointed out, if
you want to make an entire virus capsid map calculated from atomic
models you may run into trouble because Chimera will take too much
memory for the millions of atoms. Still it may be possible, but I
would reduce the atomic models to C-alpha atoms only.<br>
<br>
Tom<br>
<br>
<blockquote cite="mid:001f01cb6c13$c36f2290$0200a8c0@desktopone"
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<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><font
face="Arial"><font size="2">Hi Sir/Madam</font></font></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><o:p><font
face="Arial"><font size="2"> </font></font></o:p></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><font
face="Arial"><font size="2">I want to create models which
consist of multiple protein molecules, in which the
individual protein molecules are fused together to form a
single structure. Something like the scanning 3D maps
produced by electron microscopes. Is it possible to do
this with the Muliscale Models tool or some other tool or
method in Chimera?</font></font></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><o:p><font
face="Arial"><font size="2"> </font></font></o:p></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><font
face="Arial"><font size="2">An example of what I want to do
appears in the cutaway rotavirus illustration (Fig. 1A) in
Chen and colleagues paper, titled, “Molecular interactions
in rotavirus assembly and uncoating seen by
high-resolution cryo-EM” (PNAS, vol. 106. no. 26, p.
10644). In this illustration the yellow triangular shaped
structure are multiple VP7 molecules fused together.</font></font></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><o:p><font
face="Arial"><font size="2"> </font></font></o:p></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><font
face="Arial"><font size="2">Regards</font></font></p>
<p style="margin: 0cm 0cm 0pt;" class="MsoNormal"><font
face="Arial"><font size="2">Brian Grech</font></font></p>
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