<DIV>Thank you very much for you answer.</DIV>
<DIV>My english is not very well,Because I just come from china.</DIV>
<DIV>My major subject is gene structure,So I am not good at protein structure.</DIV>
<DIV>But ,I think this is very useful for me to analyze protein structure and asure their structure.</DIV>
<DIV>So,I must thank for you first!</DIV>
<DIV>You means:</DIV>
<DIV>If I get a homologous model structure from swissmodel which I just align with PDB stucture,i must minimize structure ,at firstĄŁ</DIV>
<DIV>But before i do minimization,I should add water or not.so this is a question.Which condition should I add water?</DIV>
<DIV>Secondly,I get a stable structure after minimization.If I want to do mutation, I should do minimization for every time mutation about this structure.</DIV>
<DIV>Thank you very much!</DIV>
<DIV>
<DIV>Thank you very much!</DIV>
<DIV>BEST WISHES FOR YOU!</DIV></DIV><br><br><span title="neteasefooter"/></span>