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<P class=MsoNormal style="MARGIN: 0in 0in 0pt"><SPAN class=post1><SPAN
style="FONT-SIZE: 10.5pt; FONT-FAMILY: Verdana"><FONT color=#333333>Dear
Listers,<?xml:namespace prefix = o ns =
"urn:schemas-microsoft-com:office:office" /><o:p></o:p></FONT></SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt"><SPAN class=post1><SPAN
style="FONT-SIZE: 10.5pt; FONT-FAMILY: Verdana"><FONT
color=#333333> <o:p></o:p></FONT></SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt"><SPAN class=post1><SPAN
style="FONT-SIZE: 10.5pt; FONT-FAMILY: Verdana"><FONT color=#333333>I have got
the CCP4 3d map of PKC created from tilted series of 2d crystals grown on lipid
monolayers. Unit cell: a=b=53A, alpha=beta=90 deg and gamma=120 deg. X-rays 3d
structures of three fragments: C1b, C2 of the regulatory domain and catalytic
domain are available from PDB data bank. When I try to dock PDB files or their
maps created with SPIDER or SITUS programs by the use of ‘fit models in maps’ or
‘fit maps in maps’ in CHIMERA, programs place this fragments into one only place
around the point that looks like a ‘center of gravity’ of CCP4 model. If I cut
off the part of model where this point is, anyway program places these fragments
into the same point out of the selected
subregion.<o:p></o:p></FONT></SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt"><SPAN class=post1><SPAN
style="FONT-SIZE: 10.5pt; FONT-FAMILY: Verdana"><FONT
color=#333333> <o:p></o:p></FONT></SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt"><SPAN class=post1><SPAN
style="FONT-SIZE: 10.5pt; FONT-FAMILY: Verdana"><FONT color=#333333>How can I
fix this problem? I understand that I have to assign the same origin of
coordinates for my model and fragments, but I do not know how to do
this.<o:p></o:p></FONT></SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt"><SPAN class=post1><SPAN
style="FONT-SIZE: 10.5pt; FONT-FAMILY: Verdana"><FONT
color=#333333> <o:p></o:p></FONT></SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt">Alexander Solodukhin, Ph.D.</P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt">Department of Anesthesiology</P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt">University of Virginia Health
System</P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt">P.O.Box 800710</P>
<P class=MsoNormal style="MARGIN: 0in 0in 0pt">Charlottesville VA 22906-0710</P>
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