<HTML><BODY style="word-wrap: break-word; -khtml-nbsp-mode: space; -khtml-line-break: after-white-space; ">Hello all,<DIV>I am trying to make a movie showing an evolution of an electron density map during an iterative optimization. For each optimization step I have one density map as volume data in xplor format. I depict the maps using the volume viewer, where I can set the number and level of the isosurface thresholds as well as their color. However, each newly opened map opens with default threshold setting, which is not suitable for my purposes. I make the movie using a command file of the type:</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>open density1.xplor</DIV><DIV>movie record</DIV><DIV>wait 10</DIV><DIV>movie stop</DIV><DIV>close 0</DIV><DIV>open density2.xplor</DIV><DIV>movie record</DIV><DIV>wait 10</DIV><DIV>movie stop</DIV><DIV>...</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>I would need to be able to control the properties of the isosurfaces of the newly opened map from the command line or from the script file, or find a way to preserve the properties set for the first map in all subsequently opened maps. The search in Chimera manual did not lead me anywhere. Could anyone give me a hint how to proceed? </DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>With best regards</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>Lukas</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>Lukas Palatinus<DIV><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><DIV>Laboratoire de Cristallographie</DIV><DIV>EPFL Lausanne</DIV><DIV><FONT class="Apple-style-span" face="Verdana"><SPAN class="Apple-style-span" style="font-family: Verdana; ">Tel.: +41 (0)21 693 0639</SPAN></FONT></DIV><DIV>mailto: <A href="mailto:palat@fzu.cz">palat@fzu.cz</A></DIV><DIV><BR class="khtml-block-placeholder"></DIV><BR class="Apple-interchange-newline"></SPAN> </DIV><BR></DIV></BODY></HTML>