<?xml version="1.0" encoding="ISO-8859-1"?>
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=ISO-8859-1" />
<title></title>
<style type="text/css">
<!--
body{margin-left:10px;margin-right:10px;margin-top:10px;margin-bottom:10px;}
-->
</style>
</head>
<body marginleft="10" marginright="10" margintop="10" marginbottom="10">
<font face="Arial" size="+0" color="#000000" style="font-family:Arial;font-size:10pt;color:#000000;">In Chimera beta 1 build 2199 on Windows XP Professional SP2, I cannot open a FASTA file that contains a single sequence. (The file in question is attached and looks to be a valid FASTA file according to the documentation I have read on the FASTA format.) What should I do about this issue?<br />
<br />
Error log:<br />
Opening F:\H Biotech Engineering\cftr.fasta<br />
Error reading F:\H Biotech Engineering\cftr.fasta:<br />
IOError: Only one sequence found in Aligned FASTA file 'F:\H Biotech Engineering\cftr.fasta'; check your alignment file for errors<br />
(see reply log for Python traceback info)<br />
Traceback (most recent call last):<br />
File "C:\Program Files\Chimera\share\__main__.py", line 59, in ?<br />
value = chimeraInit.init(sys.argv)<br />
File "C:\Program Files\Chimera\share\chimeraInit.py", line 308, in init<br />
tkgui.eventLoop()<br />
File "C:\Program Files\Chimera\share\chimera\tkgui.py", line 2757, in eventLoop<br />
app.mainloop()<br />
File "C:\Program Files\Chimera\bin\Lib\lib-tk\Tkinter.py", line 965, in mainloop<br />
self.tk.mainloop(n)<br />
File "C:\Program Files\Chimera\bin\Lib\site-packages\Pmw\Pmw_1_2\lib\PmwBase.py", line 1747, in __call__<br />
return apply(self.func, args)<br />
File "C:\Program Files\Chimera\share\chimera\baseDialog.py", line 229, in <lambda><br />
self.__buttonFuncName(txt))()<br />
File "C:\Program Files\Chimera\share\chimera\baseDialog.py", line 470, in OK<br />
self.Apply()<br />
File "C:\Program Files\Chimera\share\chimera\tkgui.py", line 160, in Apply<br />
openPath(path, ftype)<br />
File "C:\Program Files\Chimera\share\chimera\tkgui.py", line 258, in openPath<br />
mols = chimera.openModels.open(path, type=ftype)<br />
File "C:\Program Files\Chimera\share\chimera\__init__.py", line 1086, in open<br />
models = func(filename, *args, **kw)<br />
File "C:\Program Files\Chimera\share\MultAlignViewer\ChimeraExtension.py", line 33, in <lambda><br />
lambda fn, e=emo, ft=fileType: e.open(fn, ft),<br />
File "C:\Program Files\Chimera\share\MultAlignViewer\ChimeraExtension.py", line 25, in open<br />
self.module('MAViewer').MAViewer(fileName, fileType)<br />
File "C:\Program Files\Chimera\share\MultAlignViewer\MAViewer.py", line 59, in __init__<br />
seqs, fileAttrs, fileMarkups = self.readFile(<br />
File "C:\Program Files\Chimera\share\MultAlignViewer\MAViewer.py", line 761, in readFile<br />
seqs, fileAttrs, fileMarkups = parseFile(fileName, fileType)<br />
File "C:\Program Files\Chimera\share\MultAlignViewer\parse.py", line 100, in parseFile<br />
raise IOError("Only one sequence found in %s file"<br />
IOError: Only one sequence found in Aligned FASTA file 'F:\H Biotech Engineering\cftr.fasta'; check your alignment file for errors<br />
Done opening F:\H Biotech Engineering\cftr.fasta<br />
<br />
</font>
</body>
</html>