<HTML><BODY style="word-wrap: break-word; -khtml-nbsp-mode: space; -khtml-line-break: after-white-space; ">Hello. I have a stack of intensity images (8bit) taken from confocal microscopy of dapi expression in Drosophila that I'd like to visualize. I cannot figure out the format that the volume viewer requires to view the data; more specifically, none of the formats in 'registered file types' are familiar to me and I've been unable to find anything about them to sort out which format I should convert to. I have read the image sequences into matlab (3d matrix) and can write output to just about anything from there if I know the format.<DIV><BR class="khtml-block-placeholder"></DIV><DIV>Does anyone have an idea for what the best/easiest/only format I should use? A pointer to a spec on the format if it's not something trivial (like matlab 'save stack.txt varname -ascii') would be appreciated.</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>If it makes a difference, at some point soon I'd like to visualize more channels that just dapi (dll, for example) simultaneously.<DIV><DIV><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><DIV><BR class="khtml-block-placeholder"></DIV><DIV>Thanks in advance. I really do appreciate it!</DIV><DIV>-William</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>------------</DIV><DIV>William Beaver</DIV><DIV><A href="mailto:wbeaver@cs.ucsd.edu">wbeaver@cs.ucsd.edu</A></DIV><DIV><BR class="khtml-block-placeholder"></DIV><BR class="Apple-interchange-newline"></SPAN></SPAN></DIV><BR></DIV></DIV></BODY></HTML>