[Chimera-users] Blast not working

Tom Goddard goddard at sonic.net
Tue May 31 22:07:30 PDT 2022

Hi Antonio,

The error "ImportError: No module named replyobj" means Chimera cannot find its message logging module called "replyobj".  That suggests that your Chimera installation is broken, and does not appear to be a problem specifically with BLAST.  I would suggest installing the current Chimera version 1.16.

If that does not fix it, use the Report a Bug... button that should appear when this error message is shown.  Or if that button does not appear use Chimera menu entry Help / Report a Bug....


> On May 31, 2022, at 8:05 AM, Antonio Díaz Quintana via Chimera-users <chimera-users at cgl.ucsf.edu> wrote:
> Dear all, 
> Recently, I stopped being able to perform a BLAST search from the SEQUENCE tool in UCSF Chimera. Is there anything I could do to fix this?
> Log message follows:
> Web Service: BlastProtein2 is a Python wrapper that calls blastp to search nr or pdb for sequences similar to the given protein sequence
> Opal service URL: http://webservices.rbvi.ucsf.edu/opal2/services/BlastProtein2Service <http://webservices.rbvi.ucsf.edu/opal2/services/BlastProtein2Service>
> Opal job URL: http://webservices.rbvi.ucsf.edu/appBlastProtein2Service16540092552761301435050 <http://webservices.rbvi.ucsf.edu/appBlastProtein2Service16540092552761301435050>
> Traceback (most recent call last):
>   File "C:\Program Files\Chimera 1.15rc\share\chimera\tasks.py", line 179, in runStatusCB
>     task.statusCBList[0]()
>   File "C:\Program Files\Chimera 1.15rc\share\blastpdb\ParserBlastP.py", line 434, in _statusCB
>     self.finishCB(self.params, output)
>   File "C:\Program Files\Chimera 1.15rc\share\blastpdb\gui.py", line 424, in _finish
>     self.fetchColumnValues()
>   File "C:\Program Files\Chimera 1.15rc\share\blastpdb\gui.py", line 449, in fetchColumnValues
>     info = fetch_pdb_info(pdb_ids)
>   File "C:\Program Files\Chimera 1.15rc\share\blastpdb\pdbinfo.py", line 151, in fetch_pdb_info
>     import replyobj
> ImportError: No module named replyobj
> Best,
> Antonio
> -- 
> Dr. Antonio J. Díaz Quintana
> Instituto de Investigaciones Químicas
> Universidad de Sevilla - CSIC
> Avda. Americo Vespucio 49
> 41092 Sevilla
> Spain
> Phone: +34 954 48 9631
> _______________________________________________
> Chimera-users mailing list: Chimera-users at cgl.ucsf.edu
> Manage subscription: https://www.rbvi.ucsf.edu/mailman/listinfo/chimera-users

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