[Chimera-users] ASKING FOR HELP WITH INTERPRETATION OF RR DISTANCE MAPS

Elaine Meng meng at cgl.ucsf.edu
Mon Aug 8 09:10:13 PDT 2022


Dear Ernest,
Sorry, you will need to use your own scientific judgment as to how to interpret such differences.  Maybe you can find some colleagues or laboratory members with which you can discuss the differences and make hypotheses about whether any of the differences have functional or stability implications.  There may not be anything you can conclude from the measurements alone -- you may need to combine them with experimental findings for the mutant, as well as anything that is already known about how that specific protein works.
Regards,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Aug 8, 2022, at 6:12 AM, Ernest Matambo via Chimera-users <chimera-users at cgl.ucsf.edu> wrote:
> 
> Dear Sir/Madam,
> I am using Chimera 1.16 to compare two protein structures: one with mutations and a reference. I would like to calculate residue-residue distance of the substitute amino acids to the reference. I have used chimera to come up with a graph of the difference but I am struggling to interpret it. Any help will be greatly appreciated.
> Regards,
> Ernest




More information about the Chimera-users mailing list