[Chimera-users] on mrc map splitting without losing resolution

[Elaine Meng]

Dear Smith,
One way is to use the Color Zone tool (in menu under Tools... Volume Data).
<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/colorzone/colorzone.html>

Color the atoms of chain D all the same color, select the atoms of chain D and use Color Zone to color a zone around that chain.  Adjust the distance slider to the cutoff you want.  Then click the "Split Map" button to make two new maps, the part that is colored and the other part.

There are other possible ways, like masking the density map with the molecular surface of D using the "mask" command.
<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/mask.html>

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco




> On Sep 1, 2020, at 5:52 AM, Smith Lee <smith_lee123 at yahoo.com> wrote:
> 
> Dear All,
> 
> Suppose my protein complex pdb contains A,B,C and D subunits, and its mrc map is available. With Chimera, can we and how to get the mrc map for this protein complex with the part for subunit D cut out, without losing the resolution of the protein complex with subunit A, B and C?
> 
> Smith