[Chimera-users] Comparison of cryo-em maps

Elaine Meng meng at cgl.ucsf.edu
Fri Feb 28 09:00:33 PST 2020

Hi Dmitry,
Depends what you mean by “compare.”

Some thoughts are: 

(A) subtract one map from the other to see the differences: command “vop subtract”

(B) show the same isosurface level for both, Measure Volume and Area (under Tools… Volume Data) or the corresponding commands “measure volume” “measure area”


(C) morph between their isosurfaces with Morph Map tool or command “vop morph"

This tutorial has an example of morphing between two EM maps (about halfway down the page):

I hope this helps,
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Feb 28, 2020, at 1:58 AM, Dmitry Semchonok <Semchonok at gmail.com> wrote:
> Dear colleagues,
> I have similar cryo-em maps that have also some differences.
> I want to compare them.
> Is there a tool in chimera that can compare the cryo-em maps?
> Thank you!
> Sincerely,
> Dmitry

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