[Chimera-users] Subtracting the difference in EM volumes

Elaine Meng meng at cgl.ucsf.edu
Tue Aug 27 08:15:39 PDT 2019


Hi Yilog,
If I understand correctly, instead of subtracting, you could try masking one map by the isosurface of the other. See the “mask” command:

<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/mask.html>

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Aug 27, 2019, at 3:18 AM, Y. Mutum <ym337 at cam.ac.uk> wrote:
> 
> Hi 
> Is there a way to subtract the non-overlapping bits and retain only the overlapping volumes between 2 EM density maps in Chimera?
> 
> I have tried the vop subtract command, but it only seems to subtract the overlapping bits and not the inverse of it (non-overlapping bits)?
> Sorry, if I have missed it.
> Thanks
> Yilog





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