[Chimera-users] Generating symmetry mates

Vani Pande vani.pande at students.iiserpune.ac.in
Wed Oct 10 10:12:13 PDT 2018


Thank you for your help!
Vani Pande
Int PhD Biological sciences
IISER Pune
20162013


On Wed, Oct 10, 2018 at 10:24 PM Elaine Meng <meng at cgl.ucsf.edu> wrote:

> Hello Vani,
> Several possibilities if the file is from the PDB and contains symmetry
> information:
>
> Unit Cell tool (in menu under Tools… Higher-order structure)
> <
> http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/unitcell/unitcell.html
> >
>
> Multiscale Models tool (in menu under Tools… Higher-order structure)
> <
> http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multiscale/framemulti.html
> >
>
> “sym” command
> <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/sym.html>
>
> These 3 have overlapping capabilities and it might be that any/all of them
> would work in your case.  See the links for details on what kinds of
> informatino they use.
>
> You could also go directly to the RCSB PDB website and download the
> biological assembly instead of just the  asymmetric unit.
>
> If the file does not contain symmetry information, that is a different
> matter.  You might have to find another similar structure that does have
> symmetry information, then in the “sym” command tell the program to use the
> other protein’s symmetry to make the multimer of your protein.
>
> In case you didn’t know: you can use menu: Help… Search Documentation and
> look for topics like “symmetry”  and it would give the same links as I gave
> above, plus a few others (Crystal Contacts tool may also be relevant)
>
> I hope this helps,
> Elaine
> -----
> Elaine C. Meng, Ph.D.
> UCSF Chimera(X) team
> Department of Pharmaceutical Chemistry
> University of California, San Francisco
>
> > On Oct 10, 2018, at 9:29 AM, Vani Pande <
> vani.pande at students.iiserpune.ac.in> wrote:
> >
> > Hello,
> > I wanted to generate symmetry mates for the protein, I am not finding a
> way to do so in chimera.The protein is a monomer in an asymmetric unit.
>
>
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