[Chimera-users] Segment according to fitted model

[Elaine Meng]

Hi David,
If generating separate maps based on a distance cutoff from the atoms is acceptable, you could try using Color Zone (Tools… Volume Data… Color Zone).
<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/colorzone/colorzone.html>

First you’d have to color the components different colors, then select all their atoms.  In Color Zone, use the slider to pick a suitable cutoff (if any) and then click “Split Map”  to get separate  maps for the zones.

If the components are all separate models, you could color them uniquely and then select them with commands such as:
 rainbow models
select 

Alternatively, although the Segment Map tool (in same menu as Color Zone) uses the watershed method and not the atoms, you could try Segment Map and then interactively group/ungroup the resulting segments to correspond with your docked components.  I don’t know how easy or hard that might be.

<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/segger/segment.html>

I hope this helps,
Elaine
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Elaine C. Meng, Ph.D. 
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco


> On Feb 25, 2016, at 8:33 AM, David Haselbach <dhaselb at gwdg.de> wrote:
> 
> Hi,
> I do have a cryo EM map of a large complex and I fitted/modeled the individual protein components. Now I would like to segment my map according to those fitted models. Is there a possibility of doing that automatically?
> Cheers
> David