[Chimera-users] Calculation of the average isoelectric point of a proteins solvation shell

Csaba Nagy csaba.nagy at utu.fi
Thu Nov 19 06:19:46 PST 2015

Dear Chimera Developers,

I am struggling with the overexpression and purification of a cytoplasmic protein that looks to be soluble only in a very narrow pH range.
I know the average isoelectric point of the whole protein, but there would be much more informative, to know what is the average isoelectric point of the proteins solvation shell, I think. Therefore I modeled the 3D structure of the protein and I tried several other visualization programs to calculate this pI value, but I was not successful.
Could you tell me please, if there is any option to make this estimation in Chimera?
Thank you very much in advance!

Best regards,
Csaba Nagy 

Csaba I. Nagy, PhD
Postdoctoral Researcher,
PharmaCity, Itäinen Pitkäkatu 4 C, 6th floor, room 6049
Molecular Plant Biology
University of Turku
FI-20014 TURKU, Finland
Phone +358 2 333 7914
Fax +358 2 333 8075
Email  csaba.nagy at utu.fi

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