[Chimera-users] making capsid map from asymmetry unit.
meng at cgl.ucsf.edu
Tue Jun 24 10:21:28 PDT 2014
Aack! Thanks so much Giovanni, I'm glad you noticed my mistake!
On Jun 24, 2014, at 10:17 AM, Giovanni Cardone <gcardone at ucsd.edu> wrote:
> Hi Jian,
> a quick correction to Elain's reply. She mistakenly looked at a different PDB (3iyi instead of 3iyj). 3iyj contains the side chains, so you can follow her initial, exhaustive directions.
> Incidentally, also in this case the atomic model was derived from a cryo-EM map: however, the two maps deposited (EMDB 5155 and 5156) do not represent all the capsid, but just a portion of it.
> I hope this helps,
> On 06/24/2014 09:59 AM, Elaine Meng wrote:
>> Hi Jian,
>> Sorry this is a rather long answer, because I ran into several issues…
>> In general, you could
>> (1) build multimer atomic structure of capsid based on symmetry information in the PDB file, using Multiscale Models tool or command "sym" (e.g.: sym #0)<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/sym.html>
>> (2) simulate density map from atomic structure with command "molmap" (e.g.: molmap # 10) … where 10 is adjustable parameter, see the manual
>> However, for 3iyi specifically I see that the atomic structure is only the CA backbone. The process above would still make something that looked like a map, but the "densities" would be nonquantitatively low and you would have to contour at very low level to make it look reasonable.
>> From the page for 3iyi at the PDB, I see the structure is from cryoelectron microscopy (cryoEM) in the first place, so I thought it might make more sense just to get the cryoEM map from EMDB, if available.
>> To find out EMDB ID numbers, I looked at the (open access) citation given in that PDB page:
>> P22 coat protein structures reveal a novel mechanism for capsid maturation: stability without auxiliary proteins or chemical crosslinks.
>> Parent KN, Khayat R, Tu LH, Suhanovsky MM, Cortines JR, Teschke CM, Johnson JE, Baker TS.
>> Structure. 2010 Mar 10;18(3):390-401
>> … which gives accession numbers EMD-4159 (for "ExH particles," you would need to read the paper for explanation) and EMD-4150. In Chimera you can fetch EMDB entries with menu: File… Fetch by ID, database: EMDB, or command, e.g.: open emdbID:4159
>> Unfortunately, I see that those entries are not found!!! I don't know why. I also could not find them at the EMDB website.
>> So, my only idea to get a map is the 2-step process above.
>> Or, without making a map, you can show the capsid with each subunit as a "blob" using Multiscale Models (in menu under Tools… Higher-Order Structure). The images at the PDB are similar to that:
>> I hope this helps,
>> Elaine C. Meng, Ph.D.
>> UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
>> Department of Pharmaceutical Chemistry
>> University of California, San Francisco
>> On Jun 24, 2014, at 7:49 AM, "Jian Guan" <jug25 at psu.edu> wrote:
>>> Dear All,
>>> I want to make a capsid map from asymmetry unit. The PDB ID is 3iyj, the L1 protein of BPV. The BPV is a T=7, icosahedral capsid. Does anybody know how to make it? Thanks a lot.
>>> Sincerely yours,
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