[Chimera-users] Manipulating representation during MD movie

Jean Didier Pie Marechal JeanDidier.Marechal at uab.cat
Tue Feb 7 05:49:26 PST 2012


Hi guys,

I have a MD movie I generated that I want to record. However, I would need to ask on the graphical representation at given frames (i.e. frame 60) so that the system changes (i.e. change the representation en stick or add a given label). Is there a way to do so directedly? Or should I go for a command file? In this case, how would I mix the MD movie with my own succession of events?

All the best,
JD


Dr. Jean-Didier Maréchal
Lecturer
The Computational Biotechnological Chemistry Team
Unitat de Química Física
Departament de Química
Universitat Autònoma de Barcelona
Edifici C.n.
08193 Cerdanyola (Barcelona)
Tel: +34.935814936
e-mail: JeanDidier.Marechal at uab.es
www: http://asklipio.qf.uab.es

----- Missatge original -----
De: chimera-users-request at cgl.ucsf.edu
Data: Dilluns, Febrer 6, 2012 9:00 pm
Assumpte: Chimera-users Digest, Vol 106, Issue 7

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> Today's Topics:
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>   1. Rotamer copying (Ahir Pushpanath)
>   2. Re: Rotamer copying (Eric Pettersen)
> 
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> --------------------------------------------------------------------
> --
> 
> Message: 1
> Date: Mon, 6 Feb 2012 18:51:22 +0000
> From: Ahir Pushpanath <ahir29 at googlemail.com>
> To: chimera-users at cgl.ucsf.edu
> Subject: [Chimera-users] Rotamer copying
> Message-ID:
> 	<CAMVKkxyEoXbnQUo7hxa+m2-u5wLCvMJn7DDY=hzZPJEbC_OvSw at mail.gmail.com>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Hello there, I have a question regarding MODELLER in Chimera and the
> ability of chimera to adjust the model using some inbuilt features
> quickly(i.e. automatically, instead of interactively)
> 
> Firstly, I am trying to model the "holo" form of an enzyme with known
> apo-form structure. I did this usinig the two independent domains 
> of the
> apo form as a template (in a bid to get the domains accurate) and 
> for the
> holo-form closure I used a very similar homologue that has been 
> solved in
> the holo form. The results were good and I got a decent model that had
> closed up like the homolog.
> 
> However, I noticed that in the model a lot of the sidechains have 
> not been
> modelled with the right rotamer. This is odd, as I gave it the two
> independent domains as a template, so it had all the information it 
> neededto get that perfectly right.
> 
> Either way, this means, I need to manually "copy" the rotamers from 
> the apo
> form onto the "holo model", which is time consuming. I know from the
> homolog that only two or three residues in the active site change 
> rotamerupon apo-holo conformational change. So, I just want an easy 
> way to edit
> the rotamers in the holo-model to copy the rotamers of the apo-form 
> for all
> residues.
> 
> Is there an easy way to do this, is it possible to this with a 
> script? I
> have no idea how to script, mind you!
> 
> Ahir
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> Message: 2
> Date: Mon, 6 Feb 2012 11:55:54 -0800
> From: Eric Pettersen <pett at cgl.ucsf.edu>
> To: Ahir Pushpanath <ahir29 at googlemail.com>
> Cc: chimera-users at cgl.ucsf.edu
> Subject: Re: [Chimera-users] Rotamer copying
> Message-ID: <1E6304DE-DE11-49BF-A0CA-3538CE763E8D at cgl.ucsf.edu>
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> 
> I have attached a script that, if you have exactly two models open 
> and  
> they have the same residues, will copy the chi angles of the lower- 
> numbered model into the higher-numbered model.  You can run the 
> script  
> simply by opening it with File->Open.
> 
> --Eric
> 
>                         Eric Pettersen
>                         UCSF Computer Graphics Lab
>                         http://www.cgl.ucsf.edu
> 
> 
> 
> On Feb 6, 2012, at 10:51 AM, Ahir Pushpanath wrote:
> 
> > Hello there, I have a question regarding MODELLER in Chimera and 
> the  
> > ability of chimera to adjust the model using some inbuilt 
> features  
> > quickly(i.e. automatically, instead of interactively)
> >
> > Firstly, I am trying to model the "holo" form of an enzyme with  
> > known apo-form structure. I did this usinig the two independent  
> > domains of the apo form as a template (in a bid to get the 
> domains  
> > accurate) and for the holo-form closure I used a very similar  
> > homologue that has been solved in the holo form. The results were 
> 
> > good and I got a decent model that had closed up like the homolog.
> >
> > However, I noticed that in the model a lot of the sidechains have 
> 
> > not been modelled with the right rotamer. This is odd, as I gave 
> it  
> > the two independent domains as a template, so it had all the  
> > information it needed to get that perfectly right.
> >
> > Either way, this means, I need to manually "copy" the rotamers 
> from  
> > the apo form onto the "holo model", which is time consuming. I 
> know  
> > from the homolog that only two or three residues in the active 
> site  
> > change rotamer upon apo-holo conformational change. So, I just 
> want  
> > an easy way to edit the rotamers in the holo-model to copy the  
> > rotamers of the apo-form for all residues.
> >
> > Is there an easy way to do this, is it possible to this with a  
> > script? I have no idea how to script, mind you!
> >
> > Ahir
> > _______________________________________________
> > Chimera-users mailing list
> > Chimera-users at cgl.ucsf.edu
> > http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users
> 
> 
> 
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