[Chimera-users] fit molecule with Laplacian filtered map

Noboru I nobozone at gmail.com
Wed Aug 31 15:37:51 PDT 2011

This is my first time trying to apply the Laplacian filter to a
cryo-EM map in Chimera before I perform the global search to fit my
crystal structure. However, the resulting map looks like the original
map blown up into million pieces with positive and negative density
showing. I get the same effect even after I "smooth" the map with the
Gaussian filter first.
Could someone advise me on how I could use this Laplacian filtered map
information to better fit my molecule into the original EM map?
Thanks for your help in advance.

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